https://scholars.lib.ntu.edu.tw/handle/123456789/416486
標題: | Selection of aptamers for AMACR detection from DNA libraries with different primers | 作者: | LIN-CHI CHEN D. K. Yang C. F. Chou L. C. Chen |
公開日期: | 2018 | 卷: | 34 | 起(迄)頁: | 19067-190748 | 來源出版物: | Rsc Advances | 摘要: | In this work, we investigated aptamer selection against alpha-methylacyl-CoA racemase (AMACR) with three DNA libraries bearing different primers. Because of increased selection diversity, aptamers with varied structural properties, such as pre-folded, induced-folding, high and low primer-dependent conformations, were discovered. From the selection results, a dimeric aptamer was constructed and capable of detecting over-expression of AMACR in prostate cancer cell lines. In summary, this study demonstrates a library-based approach to obtain aptamers with different binding properties and provides distinct aptamers for flexible design of AMACR detection. ? The Royal Society of Chemistry 2018. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/416486 | DOI: | 10.1039/c8ra01808a | SDG/關鍵字: | Binding energy; Cell culture; Diseases; Aptamers; Binding properties; DNA library; Flexible designs; Methylacyl-CoA racemase; Over-expression; Prostate cancer cells; Selection diversity; Libraries |
顯示於: | 生物機電工程學系 |
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