https://scholars.lib.ntu.edu.tw/handle/123456789/416489
Title: | Identification of Neutralizing Monoclonal Antibodies Targeting Novel Conformational Epitopes of the Porcine Epidemic Diarrhoea Virus Spike Protein. | Authors: | CHIAN-REN JENG Chang, Chia-Yu Cheng, Ivan-Chen Chang, Yen-Chen Tsai, Pei-Shiue Lai, Seiu-Yu Huang, Yu-Liang Jeng, Chian-Ren Pang, Victor Fei IVAN-CHEN CHENG PEI-SHIUE TSAI HUI-WEN CHANG VICTOR FEI PANG YEN-CHEN CHANG |
Issue Date: | 2019 | Journal Volume: | 9 | Journal Issue: | 1 | Start page/Pages: | 2529 | Source: | Scientific reports | Abstract: | Since 2010, newly identified variants of porcine epidemic diarrhoea virus (PEDV) have caused high mortality in neonatal piglets which has devastated the swine industry. The spike (S) glycoprotein of PEDV contains multiple neutralizing epitopes and is a major target for PEDV neutralization and vaccine development. To understand the antigenicity of the new PEDV variant, we characterized the neutralizing epitopes of a new genotype 2b PEDV isolate from Taiwan, PEDV Pintung 52 (PEDV-PT), by the generation of neutralizing monoclonal antibodies (NmAbs). Two NmAbs, P4B-1, and E10E-1–10 that recognized the ectodomain of the full-length recombinant PEDV S protein and exhibited neutralizing ability against the PEDV-PT virus were selected. Recombinant truncated S proteins were used to identify the target sequences for the NmAbs and P4B-1 was shown to recognize the C-terminus of CO-26K equivalent epitope (COE) at amino acids (a.a.) 575–639 of the PEDV S. Interestingly, E10E-1–10 could recognize a novel neutralizing epitope at a.a. 435–485 within the S1 A domain of the PEDV S protein, whose importance and function are yet to be determined. Moreover, both NmAbs could not bind to linearized S proteins, indicating that only conformational epitopes are recognized. This data could improve our understanding of the antigenic structures of the PEDV S protein and facilitate future development of novel epitope-based vaccines. ? 2019, The Author(s). |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/416489 | ISSN: | 2045-2322 2045-2322 | DOI: | 10.1038/s41598-019-39844-5 | SDG/Keyword: | coronavirus spike glycoprotein; epitope; monoclonal antibody; neutralizing antibody; virus vaccine; animal; Coronavirus infection; genetics; human; immunology; isolation and purification; pathogenicity; pig; Porcine epidemic diarrhea virus; protein conformation; swine disease; Taiwan; virology; Animals; Antibodies, Monoclonal; Antibodies, Neutralizing; Coronavirus Infections; Epitopes; Humans; Porcine epidemic diarrhea virus; Protein Conformation; Spike Glycoprotein, Coronavirus; Swine; Swine Diseases; Taiwan; Viral Vaccines |
Appears in Collections: | 獸醫學系 |
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