https://scholars.lib.ntu.edu.tw/handle/123456789/416698
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Hung, C.-L. | en_US |
dc.contributor.author | SZU-HUA PAN | en_US |
dc.contributor.author | Han, C.-L. | en_US |
dc.contributor.author | Chang, C.-W. | en_US |
dc.contributor.author | Hsu, Y.-L. | en_US |
dc.contributor.author | Su, C.-H. | en_US |
dc.contributor.author | Shih, S.-C. | en_US |
dc.contributor.author | Lai, Y.-J. | en_US |
dc.contributor.author | Chiau, J.-S.C. | en_US |
dc.contributor.author | Yeh, H.-I. | en_US |
dc.contributor.author | Liu, C.-Y. | en_US |
dc.contributor.author | Lee, H.-C. | en_US |
dc.contributor.author | Lam, C.S.P. | en_US |
dc.creator | Lam, C.S.P.;Lee, H.-C.;Liu, C.-Y.;Yeh, H.-I.;Chiau, J.-S.C.;Lai, Y.-J.;Shih, S.-C.;Su, C.-H.;Hsu, Y.-L.;Chang, C.-W.;Han, C.-L.;SZU-HUA PAN;Hung, C.-L. | - |
dc.date.accessioned | 2019-08-27T07:33:21Z | - |
dc.date.available | 2019-08-27T07:33:21Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 1535-3893 | - |
dc.identifier.issn | 1535-3907 | - |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-85044110620&partnerID=MN8TOARS | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/416698 | - |
dc.description.abstract | © 2017 American Chemical Society. Diabetic cardiomyopathy is a well-recognized complication of diabetes, but its pathophysiology is unclear. We aimed to investigate the mechanisms underlying cardiac dysfunction in an elderly type 2 diabetic (T2DM) mouse model, using membrane proteomic analyses. Elderly mice were fed a high fat diet for 12 weeks to induce T2DM, and myocardial structure and function were assessed by echocardiography. Cardiomyocytes were isolated by Langendorff perfusion and subjected to iTRAQ-based quantitative membrane proteomic profiling, immunoblotting, and real-time quantitative reverse-transcriptase polymerase chain reaction. Compared to controls, elderly T2DM mice showed worse systolic function, more myocardial fibrosis and up-regulation of several heart failure markers (all p < 0.05). Cardiomyocyte membrane proteomic profiling revealed that 417 proteins had differential expressions related to perturbations in several biological processes in T2DM mice compared with the control. The most up-regulated proteins were involved in oxidative phosphorylation, whereas many down-regulated proteins were involved in cytoskeletal regulation. Differential protein expression correlated with myocardial systolic velocity by tissue Doppler. In addition, cardiomyocyte immunofluorescence staining showed greater disorganization of thick/parallel F-actin stress fibers and marked reduction in F-to-G-actin ratio in T2DM vs control (p < 0.05), which paralleled worsened myocardial systolic velocity. We concluded that cardiac contractile dysfunction in elderly T2DM mice was associated with impaired energetics and cytoskeletal disorganization. | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.relation.ispartof | Journal of Proteome Research | en_US |
dc.subject | Cytoskeletal proteins | Diabetic cardiomyopathy | F-actin | G-actin | High fat diet | ITRAQ membrane proteomic profiling | Oxidative phosphorylation | Type 2 diabetes (T2DM) | en_US |
dc.subject | Cytoskeletal proteins; Diabetic cardiomyopathy; F-actin; G-actin; High fat diet; ITRAQ membrane proteomic profiling; Oxidative phosphorylation; Type 2 diabetes (T2DM) | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | biological marker; collagen type 1; F actin; G actin; glucagon like peptide 1; glucagon like peptide 1 receptor; actin; membrane protein; animal cell; animal experiment; animal model; animal tissue; Article; cardiac muscle cell; controlled study; cytoskeleton; diabetic cardiomyopathy; echocardiography; energy transfer; heart failure; heart muscle contractility; heart muscle fibrosis; immunoblotting; immunofluorescence; isolated heart; lipid diet; liquid chromatography-mass spectrometry; male; mouse; non insulin dependent diabetes mellitus; nonhuman; oxidative phosphorylation; priority journal; protein expression; proteomics; quantitative analysis; real time polymerase chain reaction; reverse transcription polymerase chain reaction; stress fiber; systole; tissue Doppler imaging; upregulation; animal; cardiac muscle; complication; cytoskeleton; disease model; energy metabolism; fibrosis; gene expression regulation; genetics; human; metabolism; non insulin dependent diabetes mellitus; pathology; Actins; Animals; Cytoskeleton; Diabetes Mellitus, Type 2; Diabetic Cardiomyopathies; Diet, High-Fat; Disease Models, Animal; Energy Metabolism; Fibrosis; Gene Expression Regulation; Humans; Membrane Proteins; Mice; Myocardium; Myocytes, Cardiac; Proteomics | - |
dc.title | Membrane proteomics of impaired energetics and cytoskeletal disorganization in elderly diet-induced diabetic mice | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1021/acs.jproteome.7b00148 | - |
dc.identifier.doi | 60947662 | - |
dc.identifier.pmid | 28823169 | - |
dc.identifier.scopus | 2-s2.0-85044110620 | - |
dc.identifier.isi | WOS:000412789400003 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?eid=2-s2.0-85044110620&partnerID=MN8TOARS | - |
dc.relation.pages | 3504 | en_US |
dc.relation.journalvolume | 16 | en_US |
dc.relation.journalissue | 10 | en_US |
dc.identifier.external | 60947662 | - |
dc.relation.pageend | 3513 | en_US |
item.languageiso639-1 | en | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Medical Genomics and Proteomics | - |
crisitem.author.orcid | 0000-0002-0138-0434 | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 基因體暨蛋白體醫學研究所 |
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