https://scholars.lib.ntu.edu.tw/handle/123456789/416761
標題: | Tks5 and Dynamin-2 enhance actin bundle rigidity in invadosomes to promote myoblast fusion | 作者: | Chuang, Mei-Chun Lin, Shan-Shan Ohniwa, Ryosuke L. Lee, Gang-Hui Su, You-An Chang, Yu-Chen Tang, Ming-Jer SHIH-SHUN LIN |
公開日期: | 6-五月-2019 | 出版社: | ROCKEFELLER UNIV PRESS | 卷: | 218 | 期: | 5 | 起(迄)頁: | 1670 | 來源出版物: | The Journal of cell biology | 摘要: | Skeletal muscle development requires the cell-cell fusion of differentiated myoblasts to form muscle fibers. The actin cytoskeleton is known to be the main driving force for myoblast fusion; however, how actin is organized to direct intercellular fusion remains unclear. Here we show that an actin- and dynamin-2-enriched protrusive structure, the invadosome, is required for the fusion process of myogenesis. Upon differentiation, myoblasts acquire the ability to form invadosomes through isoform switching of a critical invadosome scaffold protein, Tks5. Tks5 directly interacts with and recruits dynamin-2 to the invadosome and regulates its assembly around actin filaments to strengthen the stiffness of dynamin-actin bundles and invadosomes. These findings provide a mechanistic framework for the acquisition of myogenic fusion machinery during myogenesis and reveal a novel structural function for Tks5 and dynamin-2 in organizing actin filaments in the invadosome to drive membrane fusion. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065519622&doi=10.1083%2fjcb.201809161&partnerID=40&md5=504859647f460c31dd14d034bf99765d https://scholars.lib.ntu.edu.tw/handle/123456789/416761 |
ISSN: | 0021-9525 | DOI: | 10.1083/jcb.201809161 |
顯示於: | 分子醫學研究所 |
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