https://scholars.lib.ntu.edu.tw/handle/123456789/416924
Title: | BCAS2 promotes prostate cancer cells proliferation by enhancing AR mRNA transcription and protein stability | Authors: | Kuo P.-C. Huang C.-W. Lee C.-I. Chang H.-W. Hsieh S.-W. Chung Y.-P. Lee M.-S. Huang C.-S. Tsao L.-P. Tsao Y.-P. SHOW-LI CHEN |
Issue Date: | 2015 | Publisher: | Nature Publishing Group | Journal Volume: | 112 | Journal Issue: | 2 | Start page/Pages: | 391-402 | Source: | British Journal of Cancer | Abstract: | Background: We showed previously that breast carcinoma amplified sequence 2 (BCAS2) functions as a negative regulator of p53. We also found that BCAS2 is a potential AR-associated protein. AR is essential for the growth and survival of prostate carcinoma. Therefore we characterised the correlation between BCAS2 and AR. Methods: Protein interactions were examined by GST pull-down assay and co-immunoprecipitation. Clinical prostate cancer (PCa) specimens were evaluated by immunohistochemical assay. AR transcriptional activity and LNCaP cell growth were assessed by luciferase assay and MTT assay, respectively. Results: BCAS2 expression was significantly increased in PCa. BCAS2 stabilised AR protein through both hormone-dependent and -independent manners. There are at least two mechanisms for BCAS2-mediated AR protein upregulation: One is p53-dependent. The p53 is suppressed by BCAS2 that results in increasing AR mRNA and protein expression. The other is via p53-independent inhibition of proteasome degradation. As BCAS2 can form a complex with AR and HSP90, it may function with HSP90 to stabilise AR protein from being degraded by proteasome. Conclusions: In this study, we show that BCAS2 is a novel AR-interacting protein and characterise the correlation between BCAS2 and PCa. Thus we propose that BCAS2 could be a diagnostic marker and therapeutic target for PCa. ? 2015 Cancer Research UK. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84921996125&doi=10.1038%2fbjc.2014.603&partnerID=40&md5=4c1e3cc9cc152d0f1f2b7799adc959fc https://scholars.lib.ntu.edu.tw/handle/123456789/416924 |
ISSN: | 0007-0920 | DOI: | 10.1038/bjc.2014.603 |
Appears in Collections: | 微生物學科所 |
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