https://scholars.lib.ntu.edu.tw/handle/123456789/416956
Title: | HLA-A*0201 T-cell epitopes in severe acute respiratory syndrome (SARS) coronavirus nucleocapsid and spike proteins | Authors: | Tsao Y.-P. Lin J.-Y. Jan J.-T. Leng C.-H. Chu C.-C. Yang Y.-C. SHOW-LI CHEN |
Keywords: | CTL epitope; HLA-A*0201; Nucleocapsid; SARS virus; Spike | Issue Date: | 2006 | Journal Volume: | 344 | Journal Issue: | 1 | Start page/Pages: | 63-71 | Source: | Biochemical and Biophysical Research Communications | Abstract: | The immunogenicity of HLA-A*0201-restricted cytotoxic T lymphocyte (CTL) peptide in severe acute respiratory syndrome coronavirus (SARS-CoV) nuclear capsid (N) and spike (S) proteins was determined by testing the proteins' ability to elicit a specific cellular immune response after immunization of HLA-A2.1 transgenic mice and in vitro vaccination of HLA-A2.1 positive human peripheral blood mononuclearcytes (PBMCs). First, we screened SARS N and S amino acid sequences for allele-specific motif matching those in human HLA-A2.1 MHC-I molecules. From HLA peptide binding predictions (http://thr.cit.nih.gov/molbio/hla_bind/), ten each potential N- and S-specific HLA-A2.1-binding peptides were synthesized. The high affinity HLA-A2.1 peptides were validated by T2-cell stabilization assays, with immunogenicity assays revealing peptides N223-231, N227-235, and N317-325 to be the first identified HLA-A*0201-restricted CTL epitopes of SARS-CoV N protein. In addition, previous reports identified three HLA-A*0201-restricted CTL epitopes of S protein (S978-986, S1203-1211, and S1167-1175), here we found two novel peptides S787-795 and S1042-1050 as S-specific CTL epitopes. Moreover, our identified N317-325 and S1042-1050 CTL epitopes could induce recall responses when IFN-γ stimulation of blood CD8+ T-cells revealed significant difference between normal healthy donors and SARS-recovered patients after those PBMCs were in vitro vaccinated with their cognate antigen. Our results would provide a new insight into the development of therapeutic vaccine in SARS. ? 2006 Elsevier Inc. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33646058584&doi=10.1016%2fj.bbrc.2006.03.152&partnerID=40&md5=ea75d5b59ff80056f22e0b0f71df05e1 https://scholars.lib.ntu.edu.tw/handle/123456789/416956 |
ISSN: | 0006-291X | DOI: | 10.1016/j.bbrc.2006.03.152 | SDG/Keyword: | epitope; gamma interferon; HLA A antigen; major histocompatibility antigen class 1; nucleocapsid protein; T lymphocyte antigen; virus spike protein; CpG ODN 1826; DNA; epitope; HLA A antigen; HLA A*0201 antigen; HLA A2 antigen; HLA-A*0201 antigen; membrane protein; nucleocapsid protein; nucleocapsid protein, Coronavirus; peptide; spike glycoprotein, coronavirus; virus envelope protein; virus vaccine; allele; amino terminal sequence; animal cell; article; CD8+ T lymphocyte; cellular immunity; controlled study; cytotoxic T lymphocyte; drug binding; drug efficacy; drug identification; drug screening; human; human cell; immunogenicity; mouse; nonhuman; peripheral blood mononuclear cell; prediction; priority journal; protein binding; SARS coronavirus; stimulation; T lymphocyte; transgenic mouse; validation process; animal; chemistry; genetics; immunology; molecular genetics; mononuclear cell; severe acute respiratory syndrome; Th2 cell; virus capsid; Animalia; Coronavirus; Miridae; Mus musculus; SARS coronavirus; Animals; Capsid; DNA; Epitopes, T-Lymphocyte; HLA-A Antigens; HLA-A2 Antigen; Humans; Leukocytes, Mononuclear; Membrane Glycoproteins; Mice; Mice, Transgenic; Molecular Sequence Data; Nucleocapsid Proteins; Peptides; SARS Virus; Severe Acute Respiratory Syndrome; T-Lymphocytes, Cytotoxic; Th2 Cells; Viral Envelope Proteins; Viral Vaccines |
Appears in Collections: | 微生物學科所 |
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