https://scholars.lib.ntu.edu.tw/handle/123456789/417147
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hsieh P.-F. | en_US |
dc.contributor.author | Wu M.-C. | en_US |
dc.contributor.author | Yang F.-L. | en_US |
dc.contributor.author | Chen C.-T. | en_US |
dc.contributor.author | Lou T.-C. | en_US |
dc.contributor.author | Chen Y.-Y. | en_US |
dc.contributor.author | Wu S.-H. | en_US |
dc.contributor.author | JIN-CHUAN SHEU | en_US |
dc.contributor.author | JIN-TOWN WANG | en_US |
dc.creator | Wang, Jin-Town;Sheu J.-C.;Wu S.-H.;Chen Y.-Y.;Lou T.-C.;Chen C.-T.;Yang F.-L.;Wu M.-C.;Hsieh P.-F. | - |
dc.date.accessioned | 2019-09-02T08:58:21Z | - |
dc.date.available | 2019-09-02T08:58:21Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84987836155&doi=10.3389%2ffmicb.2014.00608&partnerID=40&md5=0b03caad0ff2ace167a1f1289de4eca7 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/417147 | - |
dc.description.abstract | In the O1 strain of Klebsiella, the lipopolysaccharide (LPS) O-antigen is composed of D-galactan I and D-galactan II. Although the composition of the O1 antigen of Klebsiella was resolved more than two decades, the genetic locus involved in the biosynthesis of D-galactan II and the role of D-galactan II in bacterial pathogenesis remain unclear. Here, we report the identification of the D-galactan II-synthesizing genes by screening a transposon mutant library of an acapsulated Klebsiella pneumoniae O1 strain with bacteriophage. K. pneumoniae strain deleted for wbbY exhibited abrogated D-galactan II production; altered serum resistance and attenuation of virulence. Serologic analysis of K. pneumoniae clinical isolates demonstrated that D-galactan II was more prevalent in community-acquired pyogenic liver abscess (PLA)-causing strains than in non-tissue-invasive strains. WbbY homologs, WbbZ homologs, and lipopolysaccharide structures based on D-galactan II also were present in several Gram-negative bacteria. Immunization of mice with the magA-mutant (K- 1 O1) (that is, with a LPS D-galactan II-producing strain) provided protection against infection with an O1:K2 PLA strain. Our findings indicate that both WbbY and WbbZ homologs are sufficient for the synthesis of D-galactan II. D-galactan II represents an immunodominant antigen; is conserved among multiple species of Gram-negative bacteria and could be a useful vaccine candidate. ? 2014 Hsieh, Wu, Yang, Chen, Lou, Chen, Wu, Sheu and Wang. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Frontiers Media S.A. | en_US |
dc.relation.ispartof | Frontiers in Microbiology | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | antiD Gal II immunoglobulin G; bacterial antigen; DNA homolog; galactan; galactan II; glycosyltransferase; immunoglobulin G; Klebsiella pneumoniae O1 antigen; pACYC184 derived plasmid; plasmid vector; unclassified drug; animal experiment; animal model; animal tissue; Article; bacterial colonization; bacterial gene; bacterial virulence; bacteriophage; bacterium isolation; colony forming unit; controlled study; DNA sequence; female; gene deletion; gene locus; immunization; Klebsiella pneumoniae; mouse; mutant; nonhuman; nuclear magnetic resonance; nucleotide sequence; polymerase chain reaction; pyogenic liver abscess; transposon; WbbY gene; WbbZ gene | - |
dc.title | D-galactan II is an immunodominant antigen in O1 lipopolysaccharide and affects virulence in Klebsiella pneumoniae: Implication in vaccine design | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.3389/fmicb.2014.00608 | - |
dc.identifier.scopus | 2-s2.0-84987836155 | - |
dc.relation.journalvolume | 5 | en_US |
dc.relation.journalissue | NOV | en_US |
item.languageiso639-1 | en_US | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Microbiology | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.orcid | 0000-0002-2503-7139 | - |
crisitem.author.orcid | 0000-0002-1595-6801 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
Appears in Collections: | 微生物學科所 |
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