https://scholars.lib.ntu.edu.tw/handle/123456789/417147
標題: | D-galactan II is an immunodominant antigen in O1 lipopolysaccharide and affects virulence in Klebsiella pneumoniae: Implication in vaccine design | 作者: | Hsieh P.-F. Wu M.-C. Yang F.-L. Chen C.-T. Lou T.-C. Chen Y.-Y. Wu S.-H. JIN-CHUAN SHEU JIN-TOWN WANG |
公開日期: | 2014 | 出版社: | Frontiers Media S.A. | 卷: | 5 | 期: | NOV | 來源出版物: | Frontiers in Microbiology | 摘要: | In the O1 strain of Klebsiella, the lipopolysaccharide (LPS) O-antigen is composed of D-galactan I and D-galactan II. Although the composition of the O1 antigen of Klebsiella was resolved more than two decades, the genetic locus involved in the biosynthesis of D-galactan II and the role of D-galactan II in bacterial pathogenesis remain unclear. Here, we report the identification of the D-galactan II-synthesizing genes by screening a transposon mutant library of an acapsulated Klebsiella pneumoniae O1 strain with bacteriophage. K. pneumoniae strain deleted for wbbY exhibited abrogated D-galactan II production; altered serum resistance and attenuation of virulence. Serologic analysis of K. pneumoniae clinical isolates demonstrated that D-galactan II was more prevalent in community-acquired pyogenic liver abscess (PLA)-causing strains than in non-tissue-invasive strains. WbbY homologs, WbbZ homologs, and lipopolysaccharide structures based on D-galactan II also were present in several Gram-negative bacteria. Immunization of mice with the magA-mutant (K- 1 O1) (that is, with a LPS D-galactan II-producing strain) provided protection against infection with an O1:K2 PLA strain. Our findings indicate that both WbbY and WbbZ homologs are sufficient for the synthesis of D-galactan II. D-galactan II represents an immunodominant antigen; is conserved among multiple species of Gram-negative bacteria and could be a useful vaccine candidate. ? 2014 Hsieh, Wu, Yang, Chen, Lou, Chen, Wu, Sheu and Wang. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84987836155&doi=10.3389%2ffmicb.2014.00608&partnerID=40&md5=0b03caad0ff2ace167a1f1289de4eca7 https://scholars.lib.ntu.edu.tw/handle/123456789/417147 |
ISSN: | 1664-302X | DOI: | 10.3389/fmicb.2014.00608 | SDG/關鍵字: | antiD Gal II immunoglobulin G; bacterial antigen; DNA homolog; galactan; galactan II; glycosyltransferase; immunoglobulin G; Klebsiella pneumoniae O1 antigen; pACYC184 derived plasmid; plasmid vector; unclassified drug; animal experiment; animal model; animal tissue; Article; bacterial colonization; bacterial gene; bacterial virulence; bacteriophage; bacterium isolation; colony forming unit; controlled study; DNA sequence; female; gene deletion; gene locus; immunization; Klebsiella pneumoniae; mouse; mutant; nonhuman; nuclear magnetic resonance; nucleotide sequence; polymerase chain reaction; pyogenic liver abscess; transposon; WbbY gene; WbbZ gene |
顯示於: | 微生物學科所 |
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