|Title:||Bioassay using blocking temperature: Interparticle interactions between biofunctionalized magnetic nanoparticles conjugated with biotargets||Authors:||Yang, H. C.
Yang, T. W.
Chen, J. M.
Liao, S. H.
Chen, K. L.
|Issue Date:||27-Mar-2017||Journal Volume:||110||Journal Issue:||13||Source:||Applied Physics Letters||Abstract:||
© 2017 Author(s). This paper reports a bioassay of alpha-fetoprotein (AFP) concentration achieved via the measurement of blocking temperature (T B ). Biofunctionalized magnetic nanoparticles (BMNs) consisting of anti-alpha-fetoprotein coated onto dextran-coated magnetic nanoparticles composed of Fe 3 O 4 were prepared and then conjugated with AFP biotargets. It was found that both the saturation magnetization and value of T B increased with the concentration of the associated AFP. Furthermore, the dependence of T B of the samples on magnetic field agreed with the interparticle interaction model. Thus, this study demonstrated a platform to detect biomarkers by characterizing T B with a sensitivity limit of 20 ppb of AFP. The promising results obtained for this bioassay can be attributed to the interparticle interactions and Néel motions of magnetic moments in the BMNs.
|Appears in Collections:||物理學系|
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