|Title:||3D resolution enhancement of deep-tissue imaging based on virtual spatial overlap modulation microscopy||Authors:||Su, I. Cheng
Lin, Yen Yin
Hsu, Kuo Jen
Shen, Po Ting
|Issue Date:||25-Jul-2016||Journal Volume:||24||Journal Issue:||15||Source:||Optics Express||Abstract:||
©2016 Optical Society of America. During the last decades, several resolution enhancement methods for optical microscopy beyond diffraction limit have been developed. Nevertheless, those hardware-based techniques typically require strong illumination, and fail to improve resolution in deep tissue. Here we develop a high-speed computational approach, three-dimensional virtual spatial overlap modulation microscopy (3D-vSPOM), which immediately solves the strong-illumination issue. By amplifying only the spatial frequency component corresponding to the un-scattered point-spread-function at focus, plus 3D nonlinear value selection, 3D-vSPOM shows significant resolution enhancement in deep tissue. Since no iteration is required, 3D-vSPOM is much faster than iterative deconvolution. Compared to non-iterative deconvolution, 3D-vSPOM does not need a priori information of point-spread-function at deep tissue, and provides much better resolution enhancement plus greatly improved noise-immune response. This method is ready to be amalgamated with two-photon microscopy or other laser scanning microscopy to enhance deep-tissue resolution.
|Appears in Collections:||物理學系|
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