|Title:||Modulated electro-hyperthermia-enhanced liposomal drug uptake by cancer cells||Authors:||Wang, Yu Shan
Tsang, Yuk Wah
Huang, Cheng Chung
Chi, Mau Shin
Yang, Kai Lin
Chi, Kwan Hwa
Chiang, Hsin Chien
Li, Wen Tyng
|Keywords:||Cancer treatment | Doxorubicin | Hyperthermia | Liposome | Micropinocytosis;Cancer treatment; Doxorubicin; Hyperthermia; Liposome; Micropinocytosis||Issue Date:||1-Jan-2019||Journal Volume:||14||Source:||International Journal of Nanomedicine||Abstract:||
© 2019 Tsang et al. Purpose: Modulated electro-hyperthermia (mEHT) stands to be a significant technological advancement in the hyperthermia field, utilizing autofocusing electromagnetic power on the cell membrane to create massive apoptosis. Since mEHT possesses the unique ability to excite cell membranes, we hypothesized that mEHT could enhance the uptake of liposomal drugs by enhancing phagocytic activity. Materials and methods: Water bath control and mEHT were used to compare the enhancement of liposome-encapsulated doxorubicin (Lipodox®) uptake by cancer cells. Cancer cells were made visible by doxorubicin fluorescence to investigate drug uptake. Viable cell yield was determined via the Trypan Blue exclusion method. Various substrates were used to investigate the mechanism of drug-uptake enhancement. The murine colon carcinoma model, CT26, was used to confirm the tissue infiltration of Lipodox® and its therapeutic effect. Results: mEHT treatment showed a significant enhancement of Lipodox® uptake of doxorubicin fluorescence compared with 37°C or 42°C water bath treatment. Tumor tissue sections also confirmed that mEHT treatment achieved the highest doxorubicin concentration in vivo (1.44±0.32 µg/g in mEHT group and 0.79±0.32 µg/g in 42°C water bath). Wortmannin was used to inhibit the macropinocytosis effect and 70 kDa dextran-FITC served as uptake substance. The uptake of dextran-FITC by cancer cells significantly increased after mEHT treatment whereas such enhancement was significantly inhibited by wortmannin. Conclusion: The result showed mEHT-induced particle-uptake through macropinocytosis. mEHT-enhanced uptake of Lipodox® may amplify the therapeutic effect of liposomal drugs. This novel finding warrants further clinical investigation.
|URI:||https://scholars.lib.ntu.edu.tw/handle/123456789/424396||ISSN:||11769114||DOI:||10.2147/IJN.S188791||metadata.dc.subject.other:||doxorubicin; wortmannin; antineoplastic antibiotic; doxorubicin; macrogol; Article; cancer cell; cancer growth; colorectal carcinoma; controlled study; drug efficacy; drug potentiation; drug screening; drug uptake; human; human cell; in vitro study; in vivo study; liposomal delivery; macropinocytosis; modulated electro hyperthermia; outcome assessment; thermotherapy; A-549 cell line; analogs and derivatives; animal; apoptosis; Bagg albino mouse; cell membrane; cell survival; colon tumor; disease model; drug effect; Hep-G2 cell line; neoplasm; particle size; pathology; pinocytosis; signal transduction; A549 Cells; Animals; Antibiotics, Antineoplastic; Apoptosis; Cell Membrane; Cell Survival; Colonic Neoplasms; Disease Models, Animal; Doxorubicin; Hep G2 Cells; Humans; Hyperthermia, Induced; Mice, Inbred BALB C; Neoplasms; Particle Size; Pinocytosis; Polyethylene Glycols; Signal Transduction
|Appears in Collections:||法律學系|
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