https://scholars.lib.ntu.edu.tw/handle/123456789/425202
標題: | Molecular Mechanism of Isocupressic Acid Supresses MA-10 Cell Steroidogenesis | 作者: | Tsui, Kuan-Hao Wang, Jyun-Yuan LEANG-SHIN WU CHIH-HSIEN CHIU |
公開日期: | 2012 | 出版社: | HINDAWI LTD | 卷: | 2012 | 來源出版物: | Evidence-based complementary and alternative medicine : eCAM | 摘要: | Consumption of ponderosa pine needles causes late-term abortions in cattle and is a serious poisonous plant problem in foothill and mountain rangelands. Isocupressic acid (IA) is the component of pine needles responsible for the abortifacient effect, its abortifacient effect may be due to inhibition of steroidogenesis. To investigate the more detail molecular mechanism, we used MA-10 cell, which is wild used to investigate molecular mechanism of steroidogenesis, to characterize the molecular mechanisms underlying the actions of IA in more detail. In this report, we focus on the function of IA on important steroidogenic genes, including steroidogenic acute regulatory protein (StAR), cytochrome P450 cholesterol side-chain cleavage (P450scc), and 3β-hydroxysteroid dehydrogenase (3β-HSD). We found that IA does not affect enzyme activities of these genes but inhibits transcription of P450scc and translation of StAR and P450scc through attenuating cAMP-PKA signaling. Thus, steroid productions of cells were suppressed. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/425202 | ISSN: | 1741-427X 1741-4288 |
DOI: | 10.1155/2012/190107 |
顯示於: | 動物科學技術學系 |
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