https://scholars.lib.ntu.edu.tw/handle/123456789/425336
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | YI-FAN JIANG | en_US |
dc.contributor.author | CHUNG-HSI CHOU | en_US |
dc.contributor.author | EN-CHUNG LIN | en_US |
dc.contributor.author | Chiu, Chih-Hsien | en_US |
dc.creator | CHIH-HSIEN CHIU;EN-CHUNG LIN;CHUNG-HSI CHOU;YI-FAN JIANG | - |
dc.date.accessioned | 2019-09-26T07:23:13Z | - |
dc.date.available | 2019-09-26T07:23:13Z | - |
dc.date.issued | 2011-02 | - |
dc.identifier.issn | 10956433 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/425336 | - |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-78650522377&partnerID=MN8TOARS | - |
dc.description.abstract | Hypoxia-inducible factor 1 (HIF-1) is a transcription factor that senses and adapts cells to hypoxic environmental conditions. HIF-1 is composed of an oxygen-regulated α subunit (HIF-1α) and a constitutively expressed β subunit (HIF-1β). Taiwan voles (Microtus kikuchii) are an endemic species in Taiwan, found only in mountainous areas greater than 2000m above sea level. In this study, the full-length HIF-1α cDNA was cloned and sequenced from liver tissues of Taiwan voles. We found that HIF-1α of Taiwan voles had high sequence similarity to HIF-1α of other species. Sequence alignment of HIF-1α functional domains indicated basic helix-loop-helix (bHLH), PER-ARNT-SIM (PAS) and C-terminal transactivation (TAD-C) domains were conserved among species, but sequence variations were found between the oxygen-dependent degradation domains (ODDD). To measure Taiwan vole HIF-1α responses to hypoxia, animals were challenged with cobalt chloride, and HIF-1α mRNA and protein expression in brain, lung, heart, liver, kidney, and muscle was assessed by quantitative RT-PCR and Western blot analysis. Upon induction of hypoxic stress with cobalt chloride, an increase in HIF-1α mRNA levels was detected in lung, heart, kidney, and muscle tissue. In contrast, protein expression levels showed greater variation between individual animals. These results suggest that the regulation of HIF-1α may be important to the Taiwan vole under cobalt chloride treatments. But more details regarding the evolutionary effect of environmental pressure on HIF-1α primary sequence, HIF-1α function and regulation in Taiwan voles remain to be identified. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE INC | en_US |
dc.relation.ispartof | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology | en_US |
dc.subject | Cloning; Cobalt chloride; Hypoxia-inducible factor; Microtus kikuchii | en_US |
dc.title | Molecular characterization of hypoxia and hypoxia-inducible factor 1 alpha (HIF-1α) from Taiwan voles (Microtus kikuchii) | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.cbpa.2010.10.003 | - |
dc.identifier.pmid | 20937407 | - |
dc.identifier.scopus | 2-s2.0-78650522377 | - |
dc.identifier.isi | WOS:000286861900001 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/78650522377 | - |
dc.relation.pages | 183 | en_US |
dc.relation.journalvolume | 158 | en_US |
dc.relation.journalissue | 2 | en_US |
dc.relation.pageend | 188 | en_US |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
crisitem.author.dept | Molecular and Comparative Pathobiology | - |
crisitem.author.dept | Veterinary Medicine | - |
crisitem.author.dept | Office of Student Affairs | - |
crisitem.author.dept | Master Program in Global Agriculture Technology and Genomic Science (Global ATGS) | - |
crisitem.author.dept | Animal Science and Technology | - |
crisitem.author.dept | Master Program in Global Agriculture Technology and Genomic Science (Global ATGS) | - |
crisitem.author.orcid | 0000-0002-1692-4385 | - |
crisitem.author.orcid | 0000-0002-4983-2786 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | Administrative Unit | - |
crisitem.author.parentorg | International College | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | International College | - |
顯示於: | 動物科學技術學系 |
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