https://scholars.lib.ntu.edu.tw/handle/123456789/425361
標題: | Three-dimensional extrusion bioprinting of single- and double-network hydrogels containing dynamic covalent crosslinks | 作者: | Wang L.L. Highley C.B. Yeh Y.-C. Galarraga J.H. Uman S. Burdick J.A. YI-CHEUN YEH |
關鍵字: | 3D printing;dynamic covalent chemistry;hydrazone;hydrogel;injectable | 公開日期: | 2018 | 卷: | 106 | 期: | 4 | 起(迄)頁: | 865-875 | 來源出版物: | Journal of Biomedical Materials Research - Part A | 摘要: | The fabrication of three-dimensional (3D) scaffolds is indispensable to tissue engineering and 3D printing is emerging as an important approach towards this. Hydrogels are often used as inks in extrusion-based 3D printing, including with encapsulated cells; however, numerous challenging requirements exist, including appropriate viscosity, the ability to stabilize after extrusion, and cytocompatibility. Here, we present a shear-thinning and self-healing hydrogel crosslinked through dynamic covalent chemistry for 3D bioprinting. Specifically, hyaluronic acid was modified with either hydrazide or aldehyde groups and mixed to form hydrogels containing a dynamic hydrazone bond. Due to their shear-thinning and self-healing properties, the hydrogels could be extruded for 3D printing of structures with high shape fidelity, stability to relaxation, and cytocompatibility with encapsulated fibroblasts (>80% viability). Forces for extrusion and filament sizes were dependent on parameters such as material concentration and needle gauge. To increase scaffold functionality, a second photocrosslinkable interpenetrating network was included that was used for orthogonal photostiffening and photopatterning through a thiol-ene reaction. Photostiffening increased the scaffold's modulus (?300%) while significantly decreasing erosion (?70%), whereas photopatterning allowed for spatial modification of scaffolds with dyes. Overall, this work introduces a simple approach to both fabricate and modify 3D printed scaffolds. ? 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 865¡V875, 2018. ? 2018 Wiley Periodicals, Inc. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/425361 | ISSN: | 15493296 | DOI: | 10.1002/jbm.a.36323 |
顯示於: | 高分子科學與工程學研究所 |
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