|Title:||Response Regulator 9 and 10 Negatively Regulate Salinity Tolerance in Rice||Authors:||Wang, Wei-Chen
|Keywords:||CRISPR/Cas9; Oryza Sativa; cytokinin signaling; ion transporter; salt stress||Issue Date:||29-Jul-2019||Source:||Plant & cell physiology||Abstract:||
Cytokinins are involved in many plant growth and development processes, which also has been shown to function in abiotic stress response. Cytokinin signaling is similar to prokaryotic two-component pathways and includes the transcriptional up-regulation of type-A response regulators (RRs) by cytokinin, which in turn act to inhibit cytokinin responses to provide a negative feedback loop. Cytokinin signaling is composed of several gene families and only a handful full of genes have been studied. Here, we demonstrated the function of two highly identical rice type-A response regulators, OsRR9 and OsRR10, which are both induced by cytokinin and repressed by salinity stress in rice. Loss-of-function osrr9/osrr10 mutants have higher salinity tolerance than wild-type rice seedlings. The transcriptomic analysis uncovered several ion transporters genes which were up-regulated in response to salt in the osrr9/osrr10 mutants relative to the wild-type seedlings. These include high-affinity potassium transporters, such as OsHKT1;1, OsHKT1;3, and OsHKT2;1, which play an important role in sodium and potassium homeostasis. In addition, disruption of OsRR9 and OsRR10 also affects the expression of multiple genes related to photosynthesis, transcription activity, and phytohormone signaling. Together, these results suggest that OsRR9 and OsRR10 function as negative regulators in rice salinity tolerance.
|Appears in Collections:||農藝學系|
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