Use Microfluidic Chips to Study the Phototaxis of Lung Cancer Cells
Journal
International journal of molecular sciences
Journal Volume
20
Journal Issue
18
Date Issued
2019-09-12
Author(s)
Abstract
Cell migration is an important process involved in wound healing, tissue development, and so on. Many studies have been conducted to explore how certain chemicals and electric fields induce cell movements in specific directions, which are phenomena termed chemotaxis and electrotaxis, respectively. However, phototaxis, the directional migration of cells or organisms toward or away from light, is rarely investigated due to the difficulty of generating a precise and controllable light gradient. In this study, we designed and fabricated a microfluidic chip for simultaneously culturing cells and generating a blue light gradient for guiding cell migration. A concentration gradient was first established inside this chip, and by illuminating it with a blue light-emitting diode (LED), a blue light gradient was generated underneath. Cell migration in response to this light stimulus was observed. It was found that lung cancer cells migrated to the dark side of the gradient, and the intracellular reactive oxygen species (ROS) was proportional to the intensity of the blue light.
Subjects
cell migration | lung cancer cells | microfluidic chips | phototaxis | reactive oxygen species
SDGs
Other Subjects
reactive oxygen metabolite; reactive oxygen metabolite; A-549 cell line; Article; blue light; cancer cell; cell migration; cell viability; controlled study; electric field; environmental stress; human; human cell; intracellular space; light intensity; lung cancer; NIH 3T3 cell line; phototaxis; animal; cell culture technique; cell motion; devices; equipment design; lab on a chip; light; lung tumor; metabolism; mouse; A549 Cells; Animals; Cell Culture Techniques; Cell Movement; Equipment Design; Humans; Lab-On-A-Chip Devices; Light; Lung Neoplasms; Mice; NIH 3T3 Cells; Reactive Oxygen Species
Type
journal article