|Title:||Structural insights into the interaction between phytoplasmal effector causing phyllody 1 and MADS transcription factors||Authors:||Ko, Tzu Ping
Cheng, Han Pin
Liao, Yi Ting
Wang, Hao Ching
Sun, Wan Ting
Shen, Bing Nan
Lin, Shin Jen
Chen, Yi Fan
|Keywords:||MADS transcription factor | Phytoplasma | Phytoplasmal effector causing phyllody 1||Issue Date:||1-Jan-2019||Source:||Plant Journal||Abstract:||
© 2019 The Authors The Plant Journal © 2019 John Wiley & Sons Ltd Phytoplasmas are bacterial plant pathogens which can induce severe symptoms including dwarfism, phyllody and virescence in an infected plant. Because phytoplasmas infect many important crops such as peanut and papaya they have caused serious agricultural losses. The phytoplasmal effector causing phyllody 1 (PHYL1) is an important phytoplasmal pathogenic factor which affects the biological function of MADS transcription factors by interacting with their K (keratin-like) domain, thus resulting in abnormal plant developments such as phyllody. Until now, lack of information on the structure of PHYL1 has prevented a detailed understanding of the binding mechanism between PHYL1 and the MADS transcription factors. Here, we present the crystal structure of PHYL1 from peanut witches’-broom phytoplasma (PHYL1PnWB). This protein was found to fold into a unique α-helical hairpin with exposed hydrophobic residues on its surface that may play an important role in its biological function. Using proteomics approaches, we propose a binding mode of PHYL1PnWB with the K domain of the MADS transcription factor SEPALLATA3 (SEP3_K) and identify the residues of PHYL1PnWB that are important for this interaction. Furthermore, using surface plasmon resonance we measure the binding strength of PHYL1PnWB proteins to SEP3_K. Lastly, based on confocal images, we found that α-helix 2 of PHYL1PnWB plays an important role in PHYL1-mediated degradation of SEP3. Taken together, these results provide a structural understanding of the specific binding mechanism between PHYL1PnWB and SEP3_K.
|Appears in Collections:||植物病理與微生物學系|
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