https://scholars.lib.ntu.edu.tw/handle/123456789/425842
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jia-Huei Cai | en_US |
dc.contributor.author | TING-FENG YEH | en_US |
dc.contributor.author | HEN-WEI WEI | en_US |
dc.contributor.author | I-HSUAN LIU | en_US |
dc.creator | I-HSUAN LIU;HEN-WEI WEI;TING-FENG YEH;Jia-Huei Cai | - |
dc.date.accessioned | 2019-10-02T08:48:45Z | - |
dc.date.available | 2019-10-02T08:48:45Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 0032-5791 | - |
dc.identifier.issn | 1525-3171 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/425842 | - |
dc.description.abstract | © 2019 Poultry Science Association Inc. Blue-breasted quail has been recognized as a potential model animal. The aim of this study is to investigate the low-temperature-induced embryonic diapause in blue-breasted quail. To this end, the early embryonic staging in blue-breasted quail was briefly described and various incubation temperatures were tested. While the embryonic diapause in early blue-breasted quail embryos can be induced when the eggs were stored at 21°C, a lower temperature such as 16°C yielded a significantly better hatchability (P = 0.0231). Additionally, prolonged storage duration from 3, 7 to 14 d significantly reduced the hatchability (P < 0.0001). Visual examination on the unhatched eggs revealed that reduced hatchability in prolonged storage was significantly correlated with embryonic mortality during the first half of incubation period (R2 = 0.9999, P = 0.0055). High-throughput RNA sequencing with de novo assembly showed that a gene network cluster consisted of ND4, ND5, ND6, and COX3, which are components of mitochondrial respiratory complexes, was down-regulated in the cold-stored embryos, while a stress-responsive gene network cluster consisted of JUN, ATF3, and DUSP1 was up-regulated. Accordingly, cell death in the blastoderm was significantly increased as the storage duration prolonged from 3 to 10 d. Taken together, our study provided basic information on the temperature-induced embryonic diapause in blue-breasted quail. Furthermore, transcriptomic analysis sheds light for the molecular basis on how blastoderm cells respond to the prolonged cold-stress and stay diapause. | en_US |
dc.publisher | Oxford University Press ({OUP}) | en_US |
dc.relation.ispartof | Poultry Science | en_US |
dc.subject | blue-breasted quail | cell death | cold-shock | embryonic diapause | mitochondrial respiratory complex | en_US |
dc.title | Temperature-induced embryonic diapause in blue-breasted quail (Coturnix chinensis) correlates with decreased mitochondrial-respiratory network and increased stress-response network | en_US |
dc.identifier.doi | 55752978 | - |
dc.identifier.doi | https://doi.org/10.3382/ps/pez116 | - |
dc.identifier.doi | 10.3382/ps/pez116 | - |
dc.identifier.pmid | 30915476 | - |
dc.identifier.scopus | 2-s2.0-85068812547 | - |
dc.identifier.url | https://doi.org/10.3382/ps/pez116 | - |
dc.relation.journalvolume | 98 | en_US |
dc.relation.journalissue | 7 | en_US |
dc.identifier.external | 55752978 | - |
dc.relation.pageend | 2988 | en_US |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Forestry and Resource Conservation | - |
crisitem.author.dept | Animal Science and Technology | - |
crisitem.author.dept | Animal Science and Technology | - |
crisitem.author.orcid | 0000-0002-4114-6714 | - |
crisitem.author.orcid | 0000-0001-9096-1013 | - |
crisitem.author.orcid | 0000-0002-4524-3263 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
Appears in Collections: | 動物科學技術學系 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.