https://scholars.lib.ntu.edu.tw/handle/123456789/426460
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Wen, Peter J | en_US |
dc.contributor.author | Grenklo, Staffan | en_US |
dc.contributor.author | Arpino, Gianvito | en_US |
dc.contributor.author | Tan, Xinyu | en_US |
dc.contributor.author | HSIEN-SHUN LIAO | en_US |
dc.contributor.author | Heureaux, Johanna | en_US |
dc.contributor.author | Peng, Shi-Yong | en_US |
dc.contributor.author | Chiang, Hsueh-Cheng | en_US |
dc.contributor.author | Hamid, Edaeni | en_US |
dc.contributor.author | Zhao, Wei-Dong | en_US |
dc.contributor.author | Shin, Wonchul | en_US |
dc.contributor.author | Näreoja, Tuomas | en_US |
dc.contributor.author | Evergren, Emma | en_US |
dc.contributor.author | Jin, Yinghui | en_US |
dc.contributor.author | Karlsson, Roger | en_US |
dc.contributor.author | Ebert, Steven N | en_US |
dc.contributor.author | Jin, Albert | en_US |
dc.contributor.author | Liu, Allen P | en_US |
dc.contributor.author | Shupliakov, Oleg | en_US |
dc.contributor.author | Wu, Ling-Gang | en_US |
dc.creator | Wu, Ling-Gang;Shupliakov, Oleg;Liu, Allen P;Jin, Albert;Ebert, Steven N;Karlsson, Roger;Jin, Yinghui;Evergren, Emma;Näreoja, Tuomas;Shin, Wonchul;Zhao, Wei-Dong;Hamid, Edaeni;Chiang, Hsueh-Cheng;Peng, Shi-Yong;Heureaux, Johanna;HSIEN-SHUN LIAO;Tan, Xinyu;Arpino, Gianvito;Grenklo, Staffan;Wen, Peter J | - |
dc.date.accessioned | 2019-10-09T03:02:40Z | - |
dc.date.available | 2019-10-09T03:02:40Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/426460 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984991169&doi=10.1038%2fncomms12604&partnerID=40&md5=e7bbbe12748403def1b797cd418f9c68 | - |
dc.description.abstract | Vesicle fusion is executed via formation of an Ω-shaped structure (Ω-profile), followed by closure (kiss-and-run) or merging of the Ω-profile into the plasma membrane (full fusion). Although Ω-profile closure limits release but recycles vesicles economically, Ω-profile merging facilitates release but couples to classical endocytosis for recycling. Despite its crucial role in determining exocytosis/endocytosis modes, how Ω-profile merging is mediated is poorly understood in endocrine cells and neurons containing small ∼30-300 nm vesicles. Here, using confocal and super-resolution STED imaging, force measurements, pharmacology and gene knockout, we show that dynamic assembly of filamentous actin, involving ATP hydrolysis, N-WASP and formin, mediates Ω-profile merging by providing sufficient plasma membrane tension to shrink the Ω-profile in neuroendocrine chromaffin cells containing ∼300 nm vesicles. Actin-directed compounds also induce Ω-profile accumulation at lamprey synaptic active zones, suggesting that actin may mediate Ω-profile merging at synapses. These results uncover molecular and biophysical mechanisms underlying Ω-profile merging. | en_US |
dc.language.iso | en | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.relation.ispartof | Nature communications | en_US |
dc.subject.other | adenosine triphosphate; beta actin; F actin; neural Wiskott Aldrich syndrome protein; phospholipase C delta; actin; biochemistry; biophysics; cells and cell components; hydrolysis; membrane; physiology; protein; actin filament; actin polymerization; animal cell; Article; cell membrane; cell membrane depolarization; chromaffin cell; confocal microscopy; controlled study; electron microscopy; endocytosis; exocytosis; gene inactivation; lamprey; membrane vesicle; microtubule assembly; molecular dynamics; molecular imaging; mouse; neurosecretory cell; nonhuman; protein hydrolysis; super resolution stimulated emission depletion imaging; tension; animal; biological model; bovine; C57BL mouse; cell membrane; female; gene knockout; genetics; image processing; knockout mouse; male; membrane fusion; metabolism; microscopy; nerve cell; patch clamp technique; primary cell culture; procedures; secretory vesicle; synapse; synapse vesicle; Petromyzontidae; Actins; Animals; Cattle; Cell Membrane; Chromaffin Cells; Endocytosis; Exocytosis; Female; Gene Knockout Techniques; Image Processing, Computer-Assisted; Lampreys; Male; Membrane Fusion; Mice; Mice, Inbred C57BL; Mice, Knockout; Microscopy; Models, Biological; Molecular Imaging; Neurons; Patch-Clamp Techniques; Primary Cell Culture; Secretory Vesicles; Synapses; Synaptic Vesicles | - |
dc.title | Actin dynamics provides membrane tension to merge fusing vesicles into the plasma membrane | en_US |
dc.type | journal article | en |
dc.identifier.doi | https://api.elsevier.com/content/abstract/scopus_id/84984991169 | - |
dc.identifier.doi | 10.1038/ncomms12604 | - |
dc.identifier.pmid | 27576662 | - |
dc.identifier.scopus | 2-s2.0-84984991169 | - |
dc.identifier.isi | WOS:000391871100001 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/84984991169 | - |
dc.relation.journalvolume | 7 | en_US |
dc.relation.journalissue | 1 | en_US |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
crisitem.author.dept | Mechanical Engineering | - |
crisitem.author.orcid | 0000-0003-1338-0332 | - |
crisitem.author.parentorg | College of Engineering | - |
顯示於: | 機械工程學系 |
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