https://scholars.lib.ntu.edu.tw/handle/123456789/427061
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | JIN-CHUNG SHIH | en_US |
dc.contributor.author | Lin, Hua-Heng | en_US |
dc.contributor.author | Hsiao, An-Che | en_US |
dc.contributor.author | Su, Yi-Ting | en_US |
dc.contributor.author | Tsai, Shawn | en_US |
dc.contributor.author | CHUNG-LIANG CHIEN | en_US |
dc.contributor.author | HSIU-NI KUNG | en_US |
dc.creator | HSIU-NI KUNG;CHUNG-LIANG CHIEN;Tsai, Shawn;Su, Yi-Ting;Hsiao, An-Che;Lin, Hua-Heng;JIN-CHUNG SHIH | - |
dc.date.accessioned | 2019-10-19T01:55:09Z | - |
dc.date.available | 2019-10-19T01:55:09Z | - |
dc.date.issued | 2019-05 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/427061 | - |
dc.description.abstract | Several pregnancy complications result from abnormal trophoblast invasion. The dichotomous effect of TGF-β on epithelial-mesenchymal transition (EMT) between trophoblast invasion and cancer progression remains unknown and a critical concern. We attenuated the expression of TGF-β type 1 receptor (coding by TGFBR1) with RNA interference in trophoblastic cells and significantly enhanced the trophoblastic invasion. Analysis of microRNA profiles in trophoblasts indicated microRNA-7 as a key molecule linking TGF-β with the negative regulation of trophoblast invasion. We then attenuated TGFBR1 and miR-7 transcription by transducing either short hairpin RNA targeting TGFBR1 or anti-miR-7-locked nucleonic acid, and we observed an up-regulation of EMT-related transcription factors (TFs) and their downstream effectors, causing a mesenchymal transition of trophoblasts. Conversely, overexpression of TGFBR1 or miR-7 led to the epithelial transition of trophoblasts. Our results showed that TGF-β-induced miR-7 expression negatively modulated the TGF-β-SMAD family member 2-mediated EMT pathway via targeting EMT-related TFs and down-regulating their mesenchymal markers. These findings possibly explain, at least in part, why TGF-β exerts an opposite effect on EMT during trophoblast invasion and cancer progression.-Shih, J.-C., Lin, H.-H., Hsiao, A.-C., Su, Y.-T., Tsai, S., Chien, C.-L., Kung, H.-N. Unveiling the role of microRNA-7 in linking TGF-β-Smad-mediated epithelial-mesenchymal transition with negative regulation of trophoblast invasion. | en_US |
dc.language.iso | en | en_US |
dc.publisher | FEDERATION AMER SOC EXP BIOL | en_US |
dc.relation.ispartof | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | en_US |
dc.subject | Smad2; canonical pathway; microarray; placenta accreta; preeclampsia | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | gelatinase B; locked nucleic acid; messenger RNA; microRNA; microRNA 7; nerve cell adhesion molecule; short hairpin RNA; Smad2 protein; Smad3 protein; transcription factor Slug; transcription factor Snail; transcription factor Twist; transforming growth factor beta receptor 1; transforming growth factor beta1; unclassified drug; uvomorulin; vascular endothelial cadherin; vimentin; microRNA; MIRN7 microRNA, human; Smad protein; TGFBR1 protein, human; transforming growth factor beta; Article; cancer cell; cell invasion; controlled study; down regulation; epithelial mesenchymal transition; female; gene function; gene overexpression; gene repression; gene silencing; gene targeting; HTR-8/SVneo cell line; human; human cell; mRNA expression level; placenta accreta; placenta previa; preeclampsia; priority journal; protein expression level; protein microarray; regulatory mechanism; RNA interference; TGF beta signaling; transactivation; trophoblast; upregulation; carcinogenesis; cell motion; genetics; HEK293 cell line; metabolism; pathology; physiology; trophoblast; Carcinogenesis; Cell Movement; Epithelial-Mesenchymal Transition; HEK293 Cells; Humans; MicroRNAs; Receptor, Transforming Growth Factor-beta Type I; Smad Proteins; Transforming Growth Factor beta; Trophoblasts | - |
dc.title | Unveiling the role of microRNA-7 in linking TGF-β-Smad-mediated epithelial-mesenchymal transition with negative regulation of trophoblast invasion | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1096/fj.201801898RR | - |
dc.identifier.pmid | 30789794 | - |
dc.identifier.scopus | 2-s2.0-85065504571 | - |
dc.identifier.isi | WOS:000466932600038 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85065504571 | - |
dc.relation.pages | 6281 | en_US |
dc.relation.journalvolume | 33 | en_US |
dc.relation.journalissue | 5 | en_US |
dc.relation.pageend | 6295 | en_US |
item.languageiso639-1 | en | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics&Gynecology-NTUHHC | - |
crisitem.author.dept | Anatomy and Cell Biology | - |
crisitem.author.dept | Medical Genomics and Proteomics | - |
crisitem.author.dept | Anatomy and Cell Biology | - |
crisitem.author.orcid | 0000-0002-0296-4327 | - |
crisitem.author.orcid | 0000-0001-9806-486X | - |
crisitem.author.orcid | 0000-0001-6979-8346 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | NTU Hsin-Chu Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學系 |
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