https://scholars.lib.ntu.edu.tw/handle/123456789/431140
Title: | Islet-like clusters derived from skeletal muscle-derived stem/progenitor cells for autologous transplantation to control type 1 diabetes in mice | Authors: | Lan K.-C. CHING-CHIA WANG Yen Y.-P. RONG-SEN YANG SHING-HWA LIU DING-CHENG CHAN |
Issue Date: | 2018 | Publisher: | Taylor and Francis Ltd. | Journal Volume: | 46 | Journal Issue: | sup3 | Start page/Pages: | S328-S335 | Source: | Artificial Cells, Nanomedicine and Biotechnology | Abstract: | A population of muscle-derived stem/progenitor cells (MDSPCs) contained in skeletal muscle is responsible for muscle regeneration. MDSPCs from mouse muscle have been shown to be capable of differentiating into pancreatic islet-like cells. However, the potency of MDSPCs to differentiate into functional islet-like cluster remains to be confirmed. The therapeutic potential of autologous MDSPCs transplantation on type 1 diabetes still remains unclear. Here, we investigated a four-stage method to induce the differentiation of MDSPCs into insulin-producing clusters in vitro, and tested the autologous transplantation to control type 1 diabetes in mice. MDSPCs isolated from the skeletal muscles of mice possessed the ability to form islet-like clusters through several stages of differentiation. The expressions of pancreatic progenitor-related genes, insulin, and islet-related genes were significantly upregulated in islet-like clusters determined by the quantitative reverse transcription polymerase chain reaction. The autologous islet-like clusters transplantation effectively improved hyperglycaemia and glucose intolerance and increased the survival rate in streptozotocin-induced diabetic mice without the use of immunosuppressants. Taken together, these results provide evidence that MDSPCs from murine muscle tissues are capable of differentiating into insulin-producing clusters, which possess insulin-producing ability in vitro and in vivo, and have the potential for autologous transplantation to control type 1 diabetes. ? 2018, ? 2018 Informa UK Limited, trading as Taylor & Francis Group. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050556036&doi=10.1080%2f21691401.2018.1492421&partnerID=40&md5=8a9676f4bae39283adaf721b5204e911 https://scholars.lib.ntu.edu.tw/handle/123456789/431140 |
ISSN: | 2169-1401 | DOI: | 10.1080/21691401.2018.1492421 | SDG/Keyword: | Cytology; Differentiation (calculus); Insulin; Mammals; Polymerase chain reaction; Stem cells; Transcription; Diabetes mellitus; Glucose intolerance; islet-like clusters; Muscle regeneration; Muscle-derived stem; Muscle-derived stem cells; Quantitative reverse transcription-polymerase chain reaction; Therapeutic potentials; Muscle; C peptide; hepatocyte nuclear factor 3beta; insulin; animal experiment; animal model; Article; autotransplantation; cell differentiation; controlled study; glucose intolerance; hindlimb muscle; hyperglycemia; immunocytochemistry; in vitro study; insulin blood level; insulin dependent diabetes mellitus; insulin release; male; mouse; muscle stem cell; nonhuman; oral glucose tolerance test; reverse transcription polymerase chain reaction; streptozotocin-induced diabetes mellitus; upregulation; animal; autograft; cell differentiation; experimental diabetes mellitus; Institute for Cancer Research mouse; insulin dependent diabetes mellitus; metabolism; pancreas islet; pancreas islet transplantation; pathology; skeletal myoblast; transplantation; Animals; Autografts; Cell Differentiation; Diabetes Mellitus, Experimental; Diabetes Mellitus, Type 1; Islets of Langerhans; Islets of Langerhans Transplantation; Male; Mice; Mice, Inbred ICR; Myoblasts, Skeletal |
Appears in Collections: | 毒理學研究所 |
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