https://scholars.lib.ntu.edu.tw/handle/123456789/431140
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lan K.-C. | en_US |
dc.contributor.author | CHING-CHIA WANG | en_US |
dc.contributor.author | Yen Y.-P. | en_US |
dc.contributor.author | RONG-SEN YANG | en_US |
dc.contributor.author | SHING-HWA LIU | en_US |
dc.contributor.author | DING-CHENG CHAN | en_US |
dc.creator | Lan K.-C.;Wang C.-C.;Yen Y.-P.;Yang R.-S.;Shing-Hwa Liu;Chan D.-C. | - |
dc.date.accessioned | 2019-11-12T07:36:21Z | - |
dc.date.available | 2019-11-12T07:36:21Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 2169-1401 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050556036&doi=10.1080%2f21691401.2018.1492421&partnerID=40&md5=8a9676f4bae39283adaf721b5204e911 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/431140 | - |
dc.description.abstract | A population of muscle-derived stem/progenitor cells (MDSPCs) contained in skeletal muscle is responsible for muscle regeneration. MDSPCs from mouse muscle have been shown to be capable of differentiating into pancreatic islet-like cells. However, the potency of MDSPCs to differentiate into functional islet-like cluster remains to be confirmed. The therapeutic potential of autologous MDSPCs transplantation on type 1 diabetes still remains unclear. Here, we investigated a four-stage method to induce the differentiation of MDSPCs into insulin-producing clusters in vitro, and tested the autologous transplantation to control type 1 diabetes in mice. MDSPCs isolated from the skeletal muscles of mice possessed the ability to form islet-like clusters through several stages of differentiation. The expressions of pancreatic progenitor-related genes, insulin, and islet-related genes were significantly upregulated in islet-like clusters determined by the quantitative reverse transcription polymerase chain reaction. The autologous islet-like clusters transplantation effectively improved hyperglycaemia and glucose intolerance and increased the survival rate in streptozotocin-induced diabetic mice without the use of immunosuppressants. Taken together, these results provide evidence that MDSPCs from murine muscle tissues are capable of differentiating into insulin-producing clusters, which possess insulin-producing ability in vitro and in vivo, and have the potential for autologous transplantation to control type 1 diabetes. ? 2018, ? 2018 Informa UK Limited, trading as Taylor & Francis Group. | - |
dc.language.iso | English | - |
dc.publisher | Taylor and Francis Ltd. | - |
dc.relation.ispartof | Artificial Cells, Nanomedicine and Biotechnology | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Cytology; Differentiation (calculus); Insulin; Mammals; Polymerase chain reaction; Stem cells; Transcription; Diabetes mellitus; Glucose intolerance; islet-like clusters; Muscle regeneration; Muscle-derived stem; Muscle-derived stem cells; Quantitative reverse transcription-polymerase chain reaction; Therapeutic potentials; Muscle; C peptide; hepatocyte nuclear factor 3beta; insulin; animal experiment; animal model; Article; autotransplantation; cell differentiation; controlled study; glucose intolerance; hindlimb muscle; hyperglycemia; immunocytochemistry; in vitro study; insulin blood level; insulin dependent diabetes mellitus; insulin release; male; mouse; muscle stem cell; nonhuman; oral glucose tolerance test; reverse transcription polymerase chain reaction; streptozotocin-induced diabetes mellitus; upregulation; animal; autograft; cell differentiation; experimental diabetes mellitus; Institute for Cancer Research mouse; insulin dependent diabetes mellitus; metabolism; pancreas islet; pancreas islet transplantation; pathology; skeletal myoblast; transplantation; Animals; Autografts; Cell Differentiation; Diabetes Mellitus, Experimental; Diabetes Mellitus, Type 1; Islets of Langerhans; Islets of Langerhans Transplantation; Male; Mice; Mice, Inbred ICR; Myoblasts, Skeletal | - |
dc.title | Islet-like clusters derived from skeletal muscle-derived stem/progenitor cells for autologous transplantation to control type 1 diabetes in mice | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1080/21691401.2018.1492421 | - |
dc.identifier.pmid | 30032651 | - |
dc.identifier.scopus | 2-s2.0-85050556036 | - |
dc.relation.pages | S328-S335 | - |
dc.relation.journalvolume | 46 | - |
dc.relation.journalissue | sup3 | - |
item.languageiso639-1 | English | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
crisitem.author.dept | Pediatrics | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.dept | Orthopedic Surgery-NTUH | - |
crisitem.author.dept | Orthopedic Surgery | - |
crisitem.author.dept | Toxicology | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Geriatrics and Gerontology-NTUH | - |
crisitem.author.orcid | 0000-0002-5954-5684 | - |
crisitem.author.orcid | 0000-0002-0553-4779 | - |
crisitem.author.orcid | 0000-0002-9976-1197 | - |
crisitem.author.orcid | 0000-0003-2215-2243 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 毒理學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。