https://scholars.lib.ntu.edu.tw/handle/123456789/431165
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Cheng B.-C. | en_US |
dc.contributor.author | Chen J.-T. | en_US |
dc.contributor.author | Yang S.-T. | en_US |
dc.contributor.author | Chio C.-C. | en_US |
dc.contributor.author | SHING-HWA LIU | en_US |
dc.contributor.author | Chen R.-M. | en_US |
dc.creator | Cheng B.-C.;Chen J.-T.;Yang S.-T.;Chio C.-C.;Shing-Hwa Liu;Chen R.-M. | - |
dc.date.accessioned | 2019-11-12T07:36:29Z | - |
dc.date.available | 2019-11-12T07:36:29Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 1019-6439 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013059734&doi=10.3892%2fijo.2017.3861&partnerID=40&md5=f183188645cd6d6fc84e9cb64bb9251f | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/431165 | - |
dc.description.abstract | Malignant glioma is the most aggressive brain tumor. Hypoxic condition has been explored for killing cancer stem cells or drug-resistant tumor cells. This study investigated the effects of hypoxia on autophagic death and the possible mechanisms. Exposure of human malignant glioma U87-MG cells to cobalt chloride (CoCl2) increased cellular hypoxia-inducible factor-1α levels and concurrently decreased cell viability concentration-and time-dependently. In parallel, treatment with CoCl2 suppressed proliferation of human U87-MG cells. Autophagic cells and levels of LC3-II were concentration-and time-dependently induced in human U87-MG cells after exposure to CoCl2. However, pretreatment with 3-mehyladenine (3-MA) and chloroquine, inhibitors of cell autophagy, caused significant alleviations in CoCl2-induced cell autophagy. In contrast, exposure to rapamycin, an inducer of cell autophagy, synergistically induced hypoxiainduced autophagy of U87-MG cells. Administration of human U87-MG cells with CoCl2 triggered caspase-3 activation and cell apoptosis. Interestingly, pretreatment with 3-MA and chloroquine remarkably suppressed CoCl2-induced caspase-3 activation and cell apoptosis. Application of p53 small interference (si)RNA into human U87-MG cells downregulated levels of this protein and simultaneously lowered hypoxiaand 3-MA-induced alterations in cell autophagy, apoptosis, and death. The hypoxia-induced autophagy and apoptosis of DBTRG-05MG cells were significantly lowered by 3-MA pretreatment and p53 knockdown. Therefore, the present study shows that CoCl2 treatment can induce autophagy of human glioma cells and subsequent autophagic apoptosis via a p53-dependent pathway. Hypoxia-induced autophagic apoptosis may be applied as a therapeutic strategy for treatment of glioma patients. | - |
dc.language.iso | English | - |
dc.publisher | Spandidos Publications | - |
dc.relation.ispartof | International Journal of Oncology | - |
dc.subject | Autophagic Apoptosis; Autophagy; Hypoxia; Malignant Glioma; P53 | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | caspase 3; cobalt chloride; hypoxia inducible factor 1alpha; protein p53; 3-methyladenine; adenine; antimutagenic agent; caspase 3; chloroquine; cobalt; cobalt chloride; HIF1A protein, human; hypoxia inducible factor 1alpha; MAP1LC3A protein, human; microtubule associated protein; protein p53; rapamycin; reactive oxygen metabolite; small interfering RNA; TP53 protein, human; antineoplastic activity; antiproliferative activity; apoptosis; Article; autophagy; cell hypoxia; cell survival; cell viability; concentration response; controlled study; down regulation; drug cytotoxicity; drug mechanism; enzyme activation; glioma cell; human; human cell; analogs and derivatives; apoptosis; autophagy; Brain Neoplasms; cell proliferation; drug effects; genetics; glioma; metabolism; pathology; RNA interference; tumor cell line; Adenine; Antimutagenic Agents; Apoptosis; Autophagy; Brain Neoplasms; Caspase 3; Cell Hypoxia; Cell Line, Tumor; Cell Proliferation; Cell Survival; Chloroquine; Cobalt; Glioma; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Microtubule-Associated Proteins; Reactive Oxygen Species; RNA Interference; RNA, Small Interfering; Sirolimus; Tumor Suppressor Protein p53 | - |
dc.title | Cobalt chloride treatment induces autophagic apoptosis in human glioma cells via a p53-dependent pathway | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.3892/ijo.2017.3861 | - |
dc.identifier.pmid | 28197638 | - |
dc.identifier.scopus | 2-s2.0-85013059734 | - |
dc.relation.pages | 964-974 | - |
dc.relation.journalvolume | 50 | - |
dc.relation.journalissue | 3 | - |
item.fulltext | no fulltext | - |
item.openairetype | journal article | - |
item.languageiso639-1 | English | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Toxicology | - |
crisitem.author.orcid | 0000-0002-9976-1197 | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 毒理學研究所 |
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