https://scholars.lib.ntu.edu.tw/handle/123456789/431401
Title: | Accurate differentiation of novel Staphylococcus argenteus from Staphylococcus aureus using MALDI-TOF MS | Authors: | SHEY-YING CHEN Lee, Hao Teng, Shih Hua Wang, Xiao Mei Lee, Tai Fen Huang, Yu Chung Liao, Chun Hsing LEE-JENE TENG PO-REN HSUEH |
Keywords: | Bruker Biotyper MALDI-TOF MS | classification model | matrix-assisted laser desorption/ionization-time of flight mass spectrometry | Staphylococcus argenteus | Staphylococcus aureus;Bruker Biotyper MALDI-TOF MS; classification model; matrix-assisted laser desorption/ionization-time of flight mass spectrometry; Staphylococcus argenteus; Staphylococcus aureus | Issue Date: | 1-Jul-2018 | Publisher: | FUTURE MEDICINE LTD | Journal Volume: | 13 | Journal Issue: | 9 | Start page/Pages: | 997 | Source: | Future Microbiology | Abstract: | © 2018 Future Medicine Ltd. Aim: We evaluated a Staphylococcus argenteus-specific diagnostic profile of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for accurate identification of this novel bacterium. Materials & methods: Staphylococcus argenteus was identified based on negative crtM gene and presence of specific sequence types. A classification model was generated by cluster analysis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer results and ClinProTools software for 25 S. argenteus and 25 methicillin-susceptible S. aureus (MSSA). The performance of the classification model was validated against 72 S. argenteus and 72 MSSA isolates. Results: With cluster analysis and classification model, differentiation of 72 S. argenteus from 72 MSSA had 100.0% accuracy by chemical extraction method and 87.5% sensitivity and 100.0% specificity by direct smear method. Conclusion: The classification model could accurately differentiate S. argenteus from MSSA. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/431401 | ISSN: | 17460913 | DOI: | 10.2217/fmb-2018-0015 | SDG/Keyword: | adult; Article; bacterium detection; clinical classification; controlled study; diagnostic accuracy; diagnostic test accuracy study; disease classification; gene sequence; human; major clinical study; matrix assisted laser desorption ionization time of flight mass spectrometry; methicillin susceptible Staphylococcus aureus; middle aged; nonhuman; priority journal; retrospective study; sensitivity and specificity; Staphylococcus; Staphylococcus argenteus; Staphylococcus argenteus infection; Staphylococcus infection; chemistry; classification; matrix-assisted laser desorption-ionization mass spectrometry; microbiological examination; procedures; Staphylococcus; validation study; Bacteriological Techniques; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staphylococcus |
Appears in Collections: | 醫學院附設醫院 (臺大醫院) |
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