https://scholars.lib.ntu.edu.tw/handle/123456789/434771
Title: | Bai-Hu-Jia-Ren-Shen-Tang Decoction Reduces Fatty Liver by Activating AMP-Activated Protein Kinase in Vitro and in Vivo | Authors: | CHIA-CHUAN CHANG Liu, H.-K.; Hung, T.-M.; Huang, H.-C.; Lee, I.-J.; Cheng, J.-J.; Lin, L.-C.; Huang, C. |
Issue Date: | 2015 | Journal Volume: | 2015 | Start page/Pages: | 651734 | Source: | Evidence-based Complementary and Alternative Medicine | Abstract: | Obesity and associated conditions, such as type 2 diabetes mellitus (T2DM) and nonalcoholic fatty liver disease (NAFLD), are currently a worldwide health problem. In Asian traditional medicine, Bai-Hu-Jia-Ren-Shen-Tang (BHJRST) is widely used in diabetes patients to reduce thirst. However, whether it has a therapeutic effect on T2DM or NAFLD is not known. The aim of this study was to examine whether BHJRST had a lipid-lowering effect using a HuS-E/2 cell model of fatty liver induced by palmitate and in a db/db mouse model of dyslipidemia. Incubation of HuS-E/2 cells with palmitate markedly increased lipid accumulation and expression of adipose triglyceride lipase (ATGL), which is involved in lipolysis. BHJRST significantly decreased lipid accumulation and increased ATGL levels and phosphorylation of AMP-activated protein kinase (AMPK) and its primary downstream target, acetyl-CoA carboxylase (ACC), which are involved in fatty acid oxidation. Furthermore, after twice daily oral administration for six weeks, BHJRST significantly reduced hepatic fat accumulation in db/db mice, as demonstrated by increased hepatic AMPK and ACC phosphorylation, reduced serum triglyceride levels, and reduced hepatic total lipid content. The results show that BHJRST has a lipid-lowering effect in the liver that is mediated by activation of the AMPK signaling pathway. ??? 2015 Hui-Kang Liu et al. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/434771 | DOI: | 10.1155/2015/651734 | SDG/Keyword: | acetyl coenzyme A carboxylase; Bai Hu Jia Ren Shen Tang decoction; Chinese drug; cholesterol; ginsenoside Rg 3; hydroxymethylglutaryl coenzyme A reductase kinase; palmitic acid; triacylglycerol; triacylglycerol lipase; tubulin; unclassified drug; animal experiment; Article; controlled study; dyslipidemia; enzyme activation; enzyme phosphorylation; fatty acid oxidation; fatty liver; ginseng; human; in vitro study; in vivo study; lipid storage; male; mouse; nonhuman; priority journal; reversed phase high performance liquid chromatography; rice; signal transduction; triacylglycerol blood level; Western blotting |
Appears in Collections: | 藥學系 |
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