https://scholars.lib.ntu.edu.tw/handle/123456789/434955
標題: | CXCL12/CXCR4 promotes laryngeal and hypopharyngeal squamous cell carcinoma metastasis through MMP-13-dependent invasion via the ERK1/2/AP-1 pathway | 作者: | CHING-TING TAN CHIA-YU CHU Lu Y.-C. Chang C.-C. BEEN-REN LIN Wu H.-H. Liu H.-L. Cha S.-T. Prakash E. JENG-YUH KO Kuo M.-L. |
公開日期: | 2008 | 卷: | 29 | 期: | 8 | 起(迄)頁: | 1519-1527 | 來源出版物: | Carcinogenesis | 摘要: | Laryngeal and hypopharyngeal squamous cell carcinomas (LHSCCs) are common head and neck cancers with a high propensity for lymph node (LN) and lung metastasis. Here, we report that LHSCCs express high levels of functional CXCR4 receptors, native for chemokine stromal cell-derived factor-1 (SDF-1/CXCL12). Primary tumor immunohistochemistry from LHSCC patients has revealed significant expression of CXCR4 and CXCL12. Greater expression of CXCR4 but not that of CXCL12 is correlated with LN and distant metastasis. Reverse transcription-polymerase chain reaction and western blots have demonstrated that CXCR4 messenger RNA (mRNA) and protein were expressed in LHSCC cell lines as well, but failed to detect CXCL12 mRNA expression. CXCL12 treatment enhanced extracellular signal-regulated kinase (ERK) pathway activation and the motility/ invasiveness of LHSCC cell lines, which were blocked by treatment with a CXCR4 antagonist (AMD3100) and a specific MEK inhibitor (U0126). Results show that the mRNA and protein levels of matrix metalloproteinase (MMP)-13, but not MMP-2 or MMP-9, were elevated in HEp-2 cells in response to CXCL12. Again, U0126 almost inhibited the induction of MMP-13 in HEp-2 cells by stimulating CXCL12. The transcriptional factor, c-Jun, a downstream factor of ERK pathway, was found to be readily phosphorylated and translocated to the nucleus after 10 min of exposure to CXCL12. Blockage of c-Jun activity by transfection with c-jun antisense oligodeoxynucleotide significantly decreased CXCL12-induced MMP-13 expression and cell invasion. CXCL12 seems to enhance LHSCC cell invasion through paracrine-activated CXCR4, which triggers ERK/ c-Jun-dependent MMP-13 upregulation. ? The Author 2008. Published by Oxford University Press. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-49649102137&doi=10.1093%2fcarcin%2fbgn108&partnerID=40&md5=ffde52e19a208dcece454e54658e34cd https://scholars.lib.ntu.edu.tw/handle/123456789/434955 |
ISSN: | 0143-3334 | DOI: | 10.1093/carcin/bgn108 | SDG/關鍵字: | 1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene; antisense oligonucleotide; chemokine receptor CXCR4; chemokine receptor CXCR4 antagonist; collagenase 3; gelatinase A; gelatinase B; messenger RNA; mitogen activated protein kinase 1; mitogen activated protein kinase inhibitor; plerixafor; protein c jun; stromal cell derived factor 1; transcription factor AP 1; article; cancer cell culture; cancer invasion; cell invasion; cell motility; cell nucleus; controlled study; enzyme activation; gene expression; genetic transfection; head and neck cancer; HEp 2 cell; human; human cell; hypopharynx carcinoma; immunohistochemistry; larynx carcinoma; lung metastasis; lymph node metastasis; metastasis; priority journal; protein expression; protein induction; protein localization; protein phosphorylation; reverse transcription polymerase chain reaction; squamous cell carcinoma; upregulation; Western blotting; Carcinoma, Squamous Cell; Cell Line, Tumor; Cell Movement; Chemokine CXCL12; Humans; Hypopharyngeal Neoplasms; Immunohistochemistry; Laryngeal Neoplasms; Matrix Metalloproteinase 13; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; Neoplasm Invasiveness; Neoplasm Metastasis; Phosphorylation; Polymerase Chain Reaction; Receptors, CXCR4; Transcription Factor AP-1 |
顯示於: | 醫學系 |
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