https://scholars.lib.ntu.edu.tw/handle/123456789/438051
標題: | The relationship between energy status and AMP-activated protein kinase in human H460 lung cancer cells | 作者: | Su, M. Y. Hsieh, S. Y. Lee, Y. R. Chang, M. C. Yuan, T. T. Chang, J. M. |
關鍵字: | AMPK; Energy metabolism; Lung cancer | 公開日期: | 2010 | 卷: | 28 | 期: | 7 | 起(迄)頁: | 549-554 | 來源出版物: | Cell Biochemistry ans Function | 摘要: | In cultured cells, glucose and serum provide constant sources of energy and growth factors, both of which are important for cell survival and proliferation. AMP-activated protein kinase (AMPK) plays a key role in sensing intracellular ATP levels and acts as a critical regulator of energy homeostasis. To investigate the relationship between energy status and AMPK activity in lung cancer, H460 cells were starved in either glucose-free or serum-free medium and then re-stimulated with glucose and serum, respectively. The levels of ATP and lactate and the activities of AMPK and lactate dehydrogenase (LDH) were analyzed at different time intervals. During glucose treatment, the activity of AMPK was induced by glucose and showed biphasic reaction kinetics. The ATP level was gradually increased up to 2-fold compared with that in serum treatment after 24 h and lactate level was decreased to approximately 60%. The LDH activity slightly increased and reached a peak after 6 h. During serum treatment, the activity of AMPK was suppressed and the ATP level showed a dramatic 30% increase after 1 h. In contrast, the lactate level was gradually increased and then reverted to the background level after 24 h. The activity of LDH was slightly decreased after 12 h and eventually returned to the background level. This study showed the alteration of energy status in lung cancer cells in response to altered levels of glucose and serum. We suggest that the activation of AMPK and inhibition of glycolysis might be exploited as therapeutic tactics in cancer treatment. ? 2010 John Wiley & Sons, Ltd. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/438051 | DOI: | 10.1002/cbf.1685 | SDG/關鍵字: | adenosine triphosphate; glucose; hydroxymethylglutaryl coenzyme A reductase kinase; lactate dehydrogenase; lactic acid; article; cancer cell; cancer therapy; cell proliferation; cell survival; controlled study; energy balance; energy metabolism; enzyme activity; glycolysis; homeostasis; human; human cell; lung cancer; priority journal; Adenosine Triphosphate; AMP-Activated Protein Kinases; Cell Line, Tumor; Culture Media, Serum-Free; Down-Regulation; Energy Metabolism; Glucose; Humans; Kinetics; Lactate Dehydrogenases; Lactic Acid; Lung Neoplasms; Up-Regulation |
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