https://scholars.lib.ntu.edu.tw/handle/123456789/439057
Title: | Differential integration rates of hepatitis B virus DNA in the liver of children with chronic hepatitis B virus infection and hepatocellular carcinoma | Authors: | HSIANG-PO HUANG Tsuei D.-J. Wang K.-J. Chen Y.-L. YEN-HSUAN NI YUNG-MING JENG HUEY-LING CHEN HONG-YUAN HSU YING-LIEN CHEN |
Issue Date: | 2005 | Publisher: | Blackwell Publishing | Journal Volume: | 20 | Journal Issue: | 8 | Start page/Pages: | 1206-1214 | Source: | Journal of Gastroenterology and Hepatology (Australia) | Abstract: | Background and Aim: Integration of hepatitis B virus-DNA (HBV-DNA) into the host genome, a phenomenon found frequently in hepatocellular carcinomas (HCC) and causally linked to oncogenesis, has not been well characterized in children. The aim of the present study was to determine the prevalence of HBV integration more accurately and to decide whether the integration rate varies at different stages of chronic HBV infection in children. Methods: Of 13 children with chronic hepatitis, 14 liver biopsy tissues were analyzed. One liver tissue with pure liver cirrhosis, nine non-tumor, and nine tumor liver tissues from children with HCC were analyzed by a very sensitive method, inverse polymerase chain reaction (IPCR). Results: Thirteen genuine viral-host junctional sequences from 23 patients were successfully isolated and proved that IPCR is a useful method in this context. The results also indicated that the detection rate of HBV-DNA integration increased in parallel with the progress of liver histology towards the neoplastic transformation, with 0% in the liver of chronic hepatitis, 22.2% in non-tumor livers of HCC patients, and 66.7% in tumor liver tissues of HCC patients. Conclusion: The present results indicate that integration of HBV-DNA into the host genome was rarely confirmed at the early stage of chronic hepatitis in children until the stage of HCC formation. ? 2005 Blackwell Publishing Asia Pty Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-27744583760&doi=10.1111%2fj.1440-1746.2005.03789.x&partnerID=40&md5=c4dca7ca55ef772c31da60ed1c0596e6 https://scholars.lib.ntu.edu.tw/handle/123456789/439057 |
ISSN: | 0815-9319 | DOI: | 10.1111/j.1440-1746.2005.03789.x | SDG/Keyword: | adolescent; article; child; clinical article; controlled study; DNA integration; DNA virus; female; hepatitis B; Hepatitis B virus; human; human tissue; inverse polymerase chain reaction; liver biopsy; liver cell carcinoma; liver cirrhosis; liver histology; liver tumor; male; prevalence; priority journal |
Appears in Collections: | 醫學教育暨生醫倫理學科所 |
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