https://scholars.lib.ntu.edu.tw/handle/123456789/444524
Title: | Development of a quantitative real-time RT-PCR assay for detecting Taiwan ferret badger rabies virus in ear tissue of ferret badgers and mice | Authors: | Hsu, Ai-Ping Tseng, Chun-Hsien Lu, Yi-Ta Shih, Yu-Hua CHUNG-HSI CHOU Chen, Re-Shang Tsai, Kuo-Jung Tu, Wen-Jane Cliquet, Florence HSIANG-JUNG TSAI |
Keywords: | Rabies diagnosis; Real-time RT-PCR; Taiwan ferret badger rabies | Issue Date: | 2018 | Journal Volume: | 80 | Journal Issue: | 6 | Start page/Pages: | 1012-1019 | Source: | Journal of Veterinary Medical Science | Abstract: | In 2013, the first case of Taiwan ferret badger rabies virus (RABV-TWFB) infection was reported in Formosan ferret badgers, and two genetic groups of the virus were distinguished through phylogenetic analysis. To detect RABV-TWFB using a sensitive nucleic acid-based method, a quantitative real-time reverse transcription polymerase chain reaction targeting the conserved region of both genetic groups of RABV-TWFB was developed. This method had a limit of detection (LOD) of 40 RNA copies/reaction and detected viral RNA in brain and ear tissue specimens of infected and dead Formosan ferret badgers and mice with 100% sensitivity and specificity. The mean viral RNA load detected in the ear tissue specimens of ferret badgers ranged from 3.89 × 108 to 9.73 × 108 RNA copies/g-organ, which was 111-fold to 2,220-fold lower than the concentration detected in the brain specimens, but 2,000-fold to 5,000-fold higher than the LOD of the assay. This highly sensitive technique does not require facilities or instruments complying with strict biosafety criteria. Furthermore, it is efficient, safe, and labor-saving as only ear specimens need be sampled. Therefore, it is a promising technique for epidemiological screening of Taiwan ferret badger rabies. © 2018 The Japanese Society of Veterinary Science. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/444524 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049211718&doi=10.1292%2fjvms.17-0539&partnerID=40&md5=85a0f72e7725d100b97cc28e8e950306 |
ISSN: | 0916-7250 | DOI: | 10.1292/jvms.17-0539 | SDG/Keyword: | animal; Bagg albino mouse; isolation and purification; mouse; Mustela putorius furo; Mustelidae; phylogeny; rabies; Rabies virus; reverse transcription polymerase chain reaction; Taiwan; veterinary medicine; virology |
Appears in Collections: | 獸醫學系 |
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