https://scholars.lib.ntu.edu.tw/handle/123456789/444592
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hsiao Y.-P. | en_US |
dc.contributor.author | Shen C.-C. | en_US |
dc.contributor.author | Huang C.-H. | en_US |
dc.contributor.author | Lin Y.-C. | en_US |
dc.contributor.author | TONG-RONG JAN | en_US |
dc.creator | Hsiao Y.-P.;Shen C.-C.;Huang C.-H.;Lin Y.-C.;Jan T.-R. | - |
dc.date.accessioned | 2020-01-03T06:14:05Z | - |
dc.date.available | 2020-01-03T06:14:05Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 1567-5769 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/444592 | - |
dc.description.abstract | Iron oxide nanoparticles (IONPs) have been shown to attenuate T helper (Th)1 and Th2 cell-mediated immunity in ovalbumin (OVA)-sensitized mice. The objective of this study is to investigate the effects of IONPs on the immune responses of Th17 cells, a subset of T cells involved in various inflammatory pathologies. For in vivo study, a murine model of delayed-type hypersensitivity (DTH) was employed. BALB/c mice received a single dose of IONPs (0.2–10 mg iron/kg) via the tail vein 1 h prior to ovalbumin (OVA) sensitization. Their footpads were subcutaneously challenged with OVA to induce DTH reactions. The expression of Th17 cell-related molecules in inflamed footpads were examined by immunohistochemistry. For in vitro study, OVA-primed splenocytes were directly exposed to IONPs (1–100 μg iron/mL), and then re-stimulated with OVA in culture. The expression of Th17 cell-related molecules were measured by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. IONP administration attenuated the number of interleukin (IL)-6, IL-17, the transcription factor ROR-γ and chemokine receptor 6 positive cells in OVA-challenged footpads, whereas the number of transforming growth factor-β IL-23 and chemokine (C-C motif) ligand 20 positive cells was not altered. Direct exposure of OVA-primed splenocytes to IONPs suppressed the production of IL-6 and IL-17, and the mRNA expression of IL-17 and ROR-γt. These data indicate that exposure to IONPs attenuates Th17 cell responses in vivo and in vitro. ? 2018 | - |
dc.relation.ispartof | International Immunopharmacology | - |
dc.subject | Delayed-type hypersensitivity; Iron oxide nanoparticle; Ovalbumin; Splenocytes; Th17 | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | chemokine receptor; ferucarbotran; interleukin 17; interleukin 23; interleukin 6; ovalbumin; retinoid related orphan receptor gamma; transforming growth factor beta; ultrasmall superparamagnetic iron oxide; allergen; cytokine; ferric ion; ferric oxide; immunosuppressive agent; nanoparticle; retinoid related orphan receptor gamma; animal cell; animal experiment; animal model; animal tissue; Article; cellular immunity; controlled study; delayed hypersensitivity; foot pad; immunohistochemistry; immunomodulation; in vitro study; in vivo study; male; mouse; nonhuman; priority journal; protein expression; sensitization; spleen cell; Th17 cell; Th2 cell; animal; Bagg albino mouse; cell culture; delayed hypersensitivity; disease model; drug effect; human; immunology; metabolism; T lymphocyte subpopulation; Th17 cell; Allergens; Animals; Cells, Cultured; Cytokines; Disease Models, Animal; Ferric Compounds; Humans; Hypersensitivity, Delayed; Immunosuppressive Agents; Male; Mice; Mice, Inbred BALB C; Nanoparticles; Nuclear Receptor Subfamily 1, Group F, Member 3; Ovalbumin; T-Lymphocyte Subsets; Th17 Cells | - |
dc.title | Iron oxide nanoparticles attenuate T helper 17 cell responses in vitro and in vivo | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.intimp.2018.03.007 | - |
dc.identifier.scopus | 2-s2.0-85043526461 | - |
dc.identifier.url | https://www2.scopus.com/inward/record.uri?eid=2-s2.0-85043526461&doi=10.1016%2fj.intimp.2018.03.007&partnerID=40&md5=740127445c8b1d7174c5c46bec35c848 | - |
dc.relation.pages | 32-39 | - |
dc.relation.journalvolume | 58 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Veterinary Medicine | - |
crisitem.author.orcid | 0000-0003-2771-8381 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
Appears in Collections: | 獸醫學系 |
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