https://scholars.lib.ntu.edu.tw/handle/123456789/445251
標題: | Cellulose-based diagnostic devices for diagnosing serotype-2 dengue fever in human serum | 作者: | Wang, H.-K. Tsai, C.-H. Chen, K.-H. Tang, C.-T. Leou, J.-S. Li, P.-C. Tang, Y.-L. Hsieh, H.-J. Wu, H.-C. Cheng, C.-M. HSYUE-JEN HSIEH |
關鍵字: | Cellulose-based ELISA; Dengue fever; Lateral flow immunoassays; Rapid diagnosis | 公開日期: | 2014 | 卷: | 3 | 期: | 2 | 起(迄)頁: | 187-196 | 來源出版物: | Advanced Healthcare Materials | 摘要: | Here, two types of cellulose-based in vitro diagnostic devices are demonstrated for the diagnosis of dengue virus infection in both buffer system and human serum: 1) paper-based ELISA for providing the semiquantitative information of the disease activity of serotype-2 dengue fever to healthcare persons (i.e., monitoring the disease activity with a specific serotype in single patients); 2) lateral flow immunoassays to screen for infection with serotype-2 dengue fever (i.e., rapid YES or NO diagnosis prepared for large populations, in terms of global public health). Paper-based ELISA (specific to serotype-2 dengue fever), which builds off of our previous studies and a revised previous ELISA procedure, owns multiple advantages: 1) high sensitivity (about 40 times higher than the current ELISA-based approaches, due to our therapeutic-based monoclonal antibody) and specificity (specific to dengue virus serotype-2 nonstructural protein-1 antigens); 2) tiny amount of sample and reagent used for single tests; 3) short operating duration (i.e., rapid diagnostic device); and, 4) inexpensiveness (appropriate for use in all developing and underdeveloped nations of the world). Due to the higher sensitivity and shorter operating duration of paper-based ELISA (compared with conventional ELISA, and lateral flow immunoassays also performed in this study), this study has not only been able to perform the diagnosis of dengue virus serotype-2 nonstructural protein-1 antigens in both buffer system and human serum but also to evaluate dengue virus serotype-2 envelope proteins in the buffer system, thus successfully achieving the first such use of these proteins as the target antigen for the development of diagnostic tools. These results provide a more comprehensive understanding for the genesis of dengue fever diagnostic tools (through antibody-antigen recognition). ? 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/445251 | DOI: | 10.1002/adhm.201300150 | SDG/關鍵字: | Antigen-antibody reactions; Antigens; Cellulose; Diagnostic products; Monoclonal antibodies; Viruses; Dengue fevers; Diagnostic device; Diagnostic tools; Envelope proteins; Global public health; In-vitro diagnostic; Lateral flow immunoassay; Nonstructural proteins; Diagnosis; buffer; dengue virus serotype 2 nonstructural protein 1 antigen; unclassified drug; virus antigen; virus protein; antigen antibody reaction; article; dengue; diagnostic test accuracy study; disease activity; ELISA kit; health care cost; hematology test kit; human; immunoassay; in vitro study; intermethod comparison; nonhuman; paper based ELISA kit; priority journal; protein structure; sensitivity and specificity; serotype; serotype 2 dengue fever; virus envelope; cellulose-based ELISA; dengue fever; lateral flow immunoassays; rapid diagnosis; Cellulose; Dengue; Enzyme-Linked Immunosorbent Assay; Humans; Immunoassay |
顯示於: | 化學工程學系 |
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