https://scholars.lib.ntu.edu.tw/handle/123456789/446184
標題: | Differences in water soluble non-digestible polysaccharides and anti-inflammatory activities of fruiting bodies from two cultivated Xylaria nigripes strains | 作者: | Chen C.-F. Su C.-H. Lai M.-N. Ng L.-T. LEAN-TEIK HUANG |
關鍵字: | Anti-inflammatory; Physicochemical properties; Polysaccharides; Xylaria nigripes; β-glucans | 公開日期: | 2018 | 卷: | 116 | 起(迄)頁: | 728-734 | 來源出版物: | International Journal of Biological Macromolecules | 摘要: | Polysaccharides including β-glucans are important bioactive components of mushroom. Xylaria nigripes is a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness. This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN). Results showed that both TXNP and CXNP possessed relatively similar FT-IR spectra. TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa. The monosaccharide composition of TXNP was predominantly glucose, whereas CXNP contained xylose, mannose and glucose. Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger. This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency. ? 2018 |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/446184 | ISSN: | 01418130 | DOI: | 10.1016/j.ijbiomac.2018.05.047 | SDG/關鍵字: | cyclooxygenase 2; fungal polysaccharide; glucose; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 1beta; lipopolysaccharide; mannose; nitric oxide; prostaglandin E2; tumor necrosis factor; xylose; antiinflammatory agent; autacoid; fungal polysaccharide; animal cell; antiinflammatory activity; Article; controlled study; dose response; drug conformation; drug potency; fruiting body; fungal strain; fungus culture; molecular weight; mouse; nonhuman; physical chemistry; protein expression; RAW 264.7 cell line; Xylaria nigripes; Xylariales; animal; chemistry; fruiting body; immunology; isolation and purification; macrophage; pathology; Xylariales; Animals; Anti-Inflammatory Agents; Fruiting Bodies, Fungal; Fungal Polysaccharides; Inflammation Mediators; Macrophages; Mice; RAW 264.7 Cells; Xylariales |
顯示於: | 農業化學系 |
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