https://scholars.lib.ntu.edu.tw/handle/123456789/446218
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Hsu C. | en_US |
dc.contributor.author | Wu B.-Y. | en_US |
dc.contributor.author | Chang Y.-C. | en_US |
dc.contributor.author | Chang C.-F. | en_US |
dc.contributor.author | Chiou T.-Y. | en_US |
dc.contributor.author | NAN-WEI SU | en_US |
dc.creator | Hsu C.;Wu B.-Y.;Chang Y.-C.;Chang C.-F.;Chiou T.-Y.;Su N.-W. | - |
dc.date.accessioned | 2020-01-06T09:34:21Z | - |
dc.date.available | 2020-01-06T09:34:21Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 00218561 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/446218 | - |
dc.description.abstract | The soy isoflavones daidzein (DAI) and genistein (GEN) have beneficial effects on human health. However, their oral bioavailability is hampered by their low aqueous solubility. Our previous study revealed two water-soluble phosphorylated conjugates of isoflavones, daidzein 7-O-phosphate and genistein 7-O-phosphate, generated via biotransformation by Bacillus subtilis BCRC80517 cultivated with isoflavones. In this study, two novel derivatives of isoflavones, daidzein 4′-O-phosphate and genistein 4′-O-phosphate, were identified by HPLC-ESI-MS/MS and 1H, 13C, and 31P NMR, and their biotransformation roadmaps were proposed. Primarily, isoflavone glucosides were deglycosylated and then phosphorylated predominantly into 7-O-phosphate conjugates with traces of 4′-O-phosphate conjugates. Inevitably, trace quantities of glucosides were converted into 6?-O-succinyl glucosides. GEN was more efficiently phosphorylated than DAI. Nevertheless, the presence of GEN prolonged the time until the exponential phase of cell growth, whereas the other isoflavones showed little effect on cell growth. Our findings provide new insights into the novel microbial phosphorylation of isoflavones involved in xenobiotic metabolism. ? 2017 American Chemical Society. | - |
dc.relation.ispartof | Journal of Agricultural and Food Chemistry | - |
dc.subject | Bacillus subtilis; biotransformation; isoflavone; phosphorylation; xenobiotic metabolism | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Bacteriology; Biochemistry; Bioconversion; Carbohydrates; Cell growth; Growth kinetics; Metabolism; Phosphorylation; Physiology; Aqueous solubility; Bacillus Subtilis; Beneficial effects; biotransformation; Exponential phase; Isoflavones; Oral bioavailabilities; Xenobiotic metabolism; Flavonoids; isoflavone derivative; xenobiotic agent; Bacillus subtilis; biotransformation; drug effects; electrospray mass spectrometry; growth, development and aging; high performance liquid chromatography; isolation and purification; metabolism; nuclear magnetic resonance spectroscopy; phosphorylation; Bacillus subtilis; Biotransformation; Chromatography, High Pressure Liquid; Isoflavones; Magnetic Resonance Spectroscopy; Phosphorylation; Spectrometry, Mass, Electrospray Ionization; Xenobiotics | - |
dc.title | Phosphorylation of Isoflavones by Bacillus subtilis BCRC 80517 May Represent Xenobiotic Metabolism | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1021/acs.jafc.7b04647 | - |
dc.identifier.scopus | 2-s2.0-85040308186 | - |
dc.identifier.url | https://www2.scopus.com/inward/record.uri?eid=2-s2.0-85040308186&doi=10.1021%2facs.jafc.7b04647&partnerID=40&md5=0f31e3851050654184b40b3a7e2a4456 | - |
dc.relation.pages | 127-137 | - |
dc.relation.journalvolume | 66 | - |
dc.relation.journalissue | 1 | - |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Agricultural Chemistry | - |
crisitem.author.orcid | 0000-0002-1402-7349 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
顯示於: | 農業化學系 |
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