https://scholars.lib.ntu.edu.tw/handle/123456789/446413
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lee J.-H. | en_US |
dc.contributor.author | Chen P.B. | en_US |
dc.contributor.author | Yang S.-H. | en_US |
dc.contributor.author | Cheng C.-H. | en_US |
dc.contributor.author | Chueh L.-L. | en_US |
dc.contributor.author | Pang V.F. | en_US |
dc.contributor.author | Hsiao M. | en_US |
dc.contributor.author | VICTOR FEI PANG | en_US |
dc.contributor.author | JIH-JONG LEE | en_US |
dc.contributor.author | CHUNG-TIEN LIN | en_US |
dc.creator | Lee J.-H.;Chen P.B.;Yang S.-H.;Cheng C.-H.;Chueh L.-L.;Pang V.F.;Hsiao M.;Lin C.-T. | - |
dc.date.accessioned | 2020-01-07T06:32:27Z | - |
dc.date.available | 2020-01-07T06:32:27Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0002-9645 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/446413 | - |
dc.description.abstract | Objective - To investigate the antitumor effect of the chicken anemia virus (CAV) VP3 gene in canine mammary tumor (CMT) cells. Sample populations - Established primary canine cell lines that originated from epithelial cells of resected CMTs and nonneoplastic mammary gland epithelial (MGE) cells. Procedures - Expression vectors and lentiviral vectors encoding the VP3gene from a Taiwan-Ilan isolate of CAV were used to deliver the VP3 gene into CMT cells and nonneoplastic MGE cells. Ectopic gene expression and the pro-apoptotic effect of the VP3 gene on CMT and nonneoplastic MGE cells by either transfection or viral infection were evaluated via immunofluorescence microscopy, western blot analysis, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling analysis. Results - Overexpression of the enhanced green fluorescent protein-VP3 fusion protein was detected predominantly in the nuclei of CMT cells. In contrast, the VP3 protein was localized to the cytoplasm of nonneoplastic MGE cells. Among the fusion protein-expressing CMT cells, most underwent characteristic changes of apoptosis, whereas apoptosis was not detected in fusion protein-expressing, nonneoplastic MGE cells. Induction of apoptosis by VP3 gene overexpression in CMT cells was associated with the caspase-9-, but not the caspase-8-, mediated apoptosis pathway. Conclusions and clinical relevance - These data indicate that the VP3 gene of the CAV induces apoptosis in malignant CMT cells, but not in nonneoplastic canine MGE cells. On the basis of such tumor cell-specific killing, the VP3 gene may be a promising agent for the treatment of malignant mammary gland tumors in dogs. | - |
dc.relation.ispartof | American Journal of Veterinary Research | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | caspase 8; caspase 9; green fluorescent protein; hybrid protein; lentivirus vector; protein VP3; apoptosis; article; breast epithelium; breast tumor; cell killing; cell line; cell nucleus; Circoviridae; controlled study; cytoplasm; data analysis; dog; epithelium cell; expression vector; gene expression; genetic transfection; human; human cell; immunofluorescence microscopy; nick end labeling; nonhuman; protein expression; protein localization; tumor cell; virus gene; virus infection; virus isolation; Western blotting; Animals; Apoptosis; Blotting, Western; Capsid Proteins; Cell Line, Tumor; Chicken anemia virus; Dog Diseases; Dogs; Gene Expression Regulation, Neoplastic; Gene Therapy; Genetic Vectors; In Situ Nick-End Labeling; Lentivirus; Mammary Neoplasms, Animal; Microscopy, Fluorescence; Canis familiaris; Chicken anemia virus | - |
dc.title | Effect of the VP3 gene of chicken anemia virus on canine mammary tumor cells | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.2460/ajvr.68.4.411 | - |
dc.identifier.scopus | 2-s2.0-34247500365 | - |
dc.identifier.url | https://www2.scopus.com/inward/record.uri?eid=2-s2.0-34247500365&doi=10.2460%2fajvr.68.4.411&partnerID=40&md5=c85b49ae575c106635a72c5b45533a18 | - |
dc.relation.pages | 411-422 | - |
dc.relation.journalvolume | 68 | - |
dc.relation.journalissue | 4 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Veterinary Clinical Sciences | - |
crisitem.author.dept | Veterinary Clinical Sciences | - |
crisitem.author.orcid | 0000-0002-8048-4548 | - |
crisitem.author.orcid | 0000-0002-4424-730X | - |
crisitem.author.orcid | 0000-0002-3872-152X | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
顯示於: | 臨床動物醫學研究所 |
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