https://scholars.lib.ntu.edu.tw/handle/123456789/452193
Title: | Monoclonal antibody-functionalized mesoporous silica nanoparticles (MSN) for selective targeting breast cancer cells | Authors: | Tsai C.-P. Chen C.-Y. Hung Y. FU-HSIUNG CHANG Mou C.-Y. |
Issue Date: | 2009 | Journal Volume: | 19 | Journal Issue: | 32 | Start page/Pages: | 5737-5743 | Source: | Journal of Materials Chemistry | Abstract: | In this work, we conjugate anti-HER2/neu mAb (monoclonal antibody) to green fluorescent dye loaded mesoporous silica nanoparticles (Her-Dye@MSN) through a polyethylene glycol (MW = 5000) spacer. We examine their targeting properties toward HER2/neu over-expressing breast cancer cells and their internalization into the cells. Her-Dye@MSN nanoparticles exhibit a high targeting efficiency and the specific targeting capability is strongly affected by the mAb density on the Her-Dye@MSN. The time-course of competitive experiments with free antibody indicates that Her-Dye@MSN serves as a multivalent ligand. Moreover, Her-Dye@MSNs are internalized into cells through a receptor-mediated endocytosis and may escape from endosome to cytosol. The results reported here further support the potential of MSNs as a multifunctional smart nanocarriers for cell imaging and drug delivery. ? 2009 The Royal Society of Chemistry. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-68349141561&doi=10.1039%2fb905158a&partnerID=40&md5=a829bd2156a46aadfcd8f5a00a45010d https://scholars.lib.ntu.edu.tw/handle/123456789/452193 |
ISSN: | 09599428 | DOI: | 10.1039/b905158a | SDG/Keyword: | Breast cancer cells; Cell imaging; Competitive experiments; Cytosols; Endosomes; Fluorescent dyes; Functionalized; Mesoporous silica nanoparticles; Multivalent ligands; Nanocarriers; Receptor-mediated endocytosis; Targeting capability; Drug delivery; Mesoporous materials; Monoclonal antibodies; Nanoparticles; Polyethylene glycols; Polyethylene oxides; Silica; Surface plasmon resonance; Cell membranes |
Appears in Collections: | 生物化學暨分子生物學科研究所 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.