https://scholars.lib.ntu.edu.tw/handle/123456789/45265
標題: | 淡水河口紅樹林沈積物中脫氮細菌之研究 | 作者: | 謝文陽 | 關鍵字: | 脫氮細菌;紅樹林沈積物;水筆仔;denitrifying bacteria;mangrove sediment;Kandelia candel | 公開日期: | 1998 | 出版社: | 臺北市:國立臺灣大學海洋研究所 | 摘要: | 本研究採集淡水河河口的挖仔尾、竹圍 及關渡三地皆由紅樹科之水筆仔( Kandelia candel Druce)所形成之紅樹林純林其下之 沈積物,進行其脫氮活性和脫氮細菌的計 數、分離與鑑定之探討。採得之沈積物, 利用乙炔阻斷法(acetylene blockage technique),配合硝酸鹽或有機物之添加, 及培養鹽度和溫度之改變,以檢測此些因 子對其脫氮活性表現之影響。沈積物在硝 酸鹽及葡萄糖同時添加之培養條件下,方 能測得氧化亞氮(N2O)生成;分別添加硝酸 鹽、葡萄糖或兩者皆未添加時,則無脫氮 活性表現。在不同鹽度,並同時添加硝酸 鹽和葡萄糖培養,各處之沈積物皆有脫氮 活性表現。以35 和40℃培養,三處沈積物 皆有氧化亞氮產生,而在20、25 和30℃等 較低溫度,則僅有竹圍和關渡之部份沈積 物分別在25 和30℃能測得脫氮活性。由以 上結果推論,紅樹林沉積物的現場脫氮活 性表現可能易受限於有機物或硝酸鹽之供 給不足,鹽度應非沈積物脫氮活性之限制 因子;而在夏季以外之時間,溫度可能成 為沈積物脫氮活性之限制因子。沈積物中 脫氮細菌菌數估算利用最可能數計數 (most-probable-number counts,略稱MPN counts)法,配合含有Polypepton、Bacto-yeast extract、硝酸鹽及其它必須無機化合物的液 體培養基,所估得菌數多介於104 至105 cells/g wet wt.間。從各MPN 培養系列中共 純化出二十九株具有運動能力之脫氮細 菌,根據其革蘭氏反應、外型、發酵能力、 運動性和對氯化鈉之需求等特性初步鑑 定,其中二十六株具氯化鈉需求的革蘭氏 陰性桿菌應為Alteromonas、Pseudomonas 或Deleya。其餘三株則不具氯化鈉需求, 包括二株屬於Pseudomonas 的革蘭氏陰性 桿菌及一株屬於Bacillus 的革蘭氏陽性桿 菌。 This work investigated denitrifying bacteria and their denitrifying activity in the sediment from forests of the mangrove Kandelia candel in the Tansui Estuary. The samples were collected from Watsuwe 、 Chuwei and Kuantu. Effects of nitrate, glucose, NaCl and temperature on the denitrifying (N2O-producing) activity of the sediment samples were determined by the acetylene blockage technique. Nitrous oxide (N2O) was never detectable unless the samples were supplemented with both nitrate and glucose. The levels of such potential denitrifying activity did not greatly differ in NaCl concentrations ranging from 0 to 3.5%. All the samples supplemented with both nitrate and glucose exhibited detectable activity within 12h incubation at 35 or 40℃. However, activity was not detected from any of those incubated at 20 ℃ , and those incubated at 25 or 30℃ exhibited nil or relative low levels of activity. Insufficiency of both nitrate and organic matter would be apt to restrict the magnitude of in situ denitrification in the sediment of the mangrove forests. Salinity is probably not a limiting factor for the activity, whereas low temperature in the seasons other than summer would be a main factor limiting the expression of denitrifying activity. Denitrifying bacteria distributed in the mangrove forest sediment were enumerated by the most-probable-number (MPN) method 2 in liquid broth. The counting values ranged from 104 to 105 cells/g wet wt.. Twenty-nine rods-shaped, denitrifying bacteria isolated from the sediment samples were divided into three group according to the tests of Gram reactions、fermentation、motility and salt requirement. Twenty-six salt-requiring Gram-negative strains were identified as members of the group Alteromonas- Pseudomonas- Deleya. The other three strains that did not require salt for growth were identified as species of either Pseudomonas (two strains) or Bacillus (one strain). |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/29287 | 其他識別: | 872611B002A003 | Rights: | 國立臺灣大學海洋研究所 |
顯示於: | 海洋研究所 |
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