https://scholars.lib.ntu.edu.tw/handle/123456789/452859
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Huang Y.-W. | en_US |
dc.contributor.author | Chiu C.-C. | en_US |
dc.contributor.author | JA-DER LIANG | en_US |
dc.contributor.author | Chiou L.-L. | en_US |
dc.contributor.author | GUAN-TARN HUANG | en_US |
dc.contributor.author | MING-JIUN YU | en_US |
dc.contributor.author | Lee, Hsuan-Shu | en_US |
dc.creator | Huang Y.-W.;Chiu C.-C.;Liang J.-D.;Chiou L.-L.;Huang G.-T.;Ming-Jiun Yu;Lee H.-S. | - |
dc.date.accessioned | 2020-01-22T08:52:01Z | - |
dc.date.available | 2020-01-22T08:52:01Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 19326203 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84926639380&doi=10.1371%2fjournal.pone.0122528&partnerID=40&md5=1b348f997a4af129b7bdcc532f53b836 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/452859 | - |
dc.description.abstract | An appropriate liver-specific progenitor cell marker is a stepping stone in liver regenerative medicine. Here, we report brain isoform glycogen phosphorylase (GPBB) as a novel liver progenitor cell marker. GPBB was identified in a protein complex precipitated by a monoclonal antibody Ligab generated from a rat liver progenitor cell line Lig-8. Immunoblotting results show that GPBB was expressed in two liver progenitor cell lines Lig-8 and WB-F344. The levels of GPBB expression decreased in the WB-F344 cells under sodium butyrate (SB)-induced cell differentiation, consistent with roles of GPBB as a liver progenitor cell marker. Short hairpin RNA (shRNA)-mediated GPBB knockdown followed by glucose deprivation test shows that GPBB aids in liver progenitor cell survival under low glucose conditions. Furthermore, shRNA-mediated GPBB knockdown followed by SB-induced cell differentiation shows that reducing GPBB expression delayed liver progenitor cell differentiation. We conclude that GPBB is a novel liver progenitor cell marker, which facilitates liver progenitor cell survival under low glucose conditions and cell differentiation. ? 2015 Huang et al. | en_US |
dc.publisher | Public Library of Science | en_US |
dc.relation.ispartof | PLoS ONE | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | butyric acid; glucose; glycogen phosphorylase; monoclonal antibody; monoclonal antibody Ligab; short hairpin RNA; unclassified drug; butyric acid; glycogen phosphorylase; animal cell; Article; cell differentiation; cell level; cell specificity; cell survival; complex formation; controlled study; enzyme activity; functional proteomics; gene silencing; growth regulation; hepatic progenitor cell; immunoblotting; nonhuman; protein expression; rat; animal; cell culture; cell differentiation; cell line; cytology; drug effects; enzymology; Fischer 344 rat; genetics; immunoprecipitation; liver; metabolism; stem cell; Rattus; Animals; Butyric Acid; Cell Differentiation; Cell Line; Cells, Cultured; Gene Knockdown Techniques; Glycogen Phosphorylase; Glycogen Phosphorylase, Brain Form; Immunoprecipitation; Liver; Rats; Rats, Inbred F344; Stem Cells | - |
dc.title | Brain isoform glycogen phosphorylase as a novel hepatic progenitor cell marker | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1371/journal.pone.0122528 | - |
dc.identifier.scopus | 2-s2.0-84926639380 | - |
dc.relation.journalvolume | 10 | en_US |
dc.relation.journalissue | 3 | en_US |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Biochemistry and Molecular Biology | - |
crisitem.author.dept | Medical Genomics and Proteomics | - |
crisitem.author.dept | Biotechnology | - |
crisitem.author.orcid | 0000-0002-4532-0118 | - |
crisitem.author.orcid | 0000-0001-7004-826X | - |
crisitem.author.orcid | 0000-0003-0393-4696 | - |
crisitem.author.orcid | 0000-0002-8017-0434 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
顯示於: | 生物化學暨分子生物學科研究所 |
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