https://scholars.lib.ntu.edu.tw/handle/123456789/454013
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lin B.-C. | en_US |
dc.contributor.author | Su L.-H. | en_US |
dc.contributor.author | Weng S.-C. | en_US |
dc.contributor.author | Pan Y.-J. | en_US |
dc.contributor.author | NEI-LI CHAN | en_US |
dc.contributor.author | TSAI-KUN LI | en_US |
dc.contributor.author | Wang H.-C. | en_US |
dc.contributor.author | CHIN-HUNG SUN | en_US |
dc.creator | Lin B.-C.;Su L.-H.;Weng S.-C.;Pan Y.-J.;Nei-Li Chan;Li T.-K.;Wang H.-C.;Sun C.-H. | - |
dc.date.accessioned | 2020-02-06T06:42:16Z | - |
dc.date.available | 2020-02-06T06:42:16Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1935-2727 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878514953&doi=10.1371%2fjournal.pntd.0002218&partnerID=40&md5=95e059a2774f9adf8f37e43c3060a9e2 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/454013 | - |
dc.description.abstract | The protozoan Giardia lamblia differentiates into infectious cysts within the human intestinal tract for disease transmission. Expression of the cyst wall protein (cwp) genes increases with similar kinetics during encystation. However, little is known how their gene regulation shares common mechanisms. DNA topoisomerases maintain normal topology of genomic DNA. They are necessary for cell proliferation and tissue development as they are involved in transcription, DNA replication, and chromosome condensation. A putative topoisomerase II (topo II) gene has been identified in the G. lamblia genome. We asked whether Topo II could regulate Giardia encystation. We found that Topo II was present in cell nuclei and its gene was up-regulated during encystation. Topo II has typical ATPase and DNA cleavage activity of type II topoisomerases. Mutation analysis revealed that the catalytic important Tyr residue and cleavage domain are important for Topo II function. We used etoposide-mediated topoisomerase immunoprecipitation assays to confirm the binding of Topo II to the cwp promoters in vivo. Interestingly, Topo II overexpression increased the levels of cwp gene expression and cyst formation. Microarray analysis identified up-regulation of cwp and specific vsp genes by Topo II. We also found that the type II topoisomerase inhibitor etoposide has growth inhibition effect on Giardia. Addition of etoposide significantly decreased the levels of cwp gene expression and cyst formation. Our results suggest that Topo II has been functionally conserved during evolution and that Topo II plays important roles in induction of the cwp genes, which is key to Giardia differentiation into cysts. ? 2013 Lin et al. | - |
dc.publisher | Public Library of Science | - |
dc.relation.ispartof | PLoS Neglected Tropical Diseases | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | adenosine triphosphatase; cyst wall protein; DNA topoisomerase (ATP hydrolysing); etoposide; protein Myb; protozoal protein; unclassified drug; animal cell; article; cell differentiation; cell proliferation; chromatin immunoprecipitation; controlled study; DNA binding; DNA cleavage; gel mobility shift assay; gene expression; gene expression regulation; gene isolation; Giardia lamblia; immunofluorescence test; microarray analysis; mutational analysis; nonhuman; nucleotide sequence; open reading frame; protein expression; protein localization; protein purification; real time polymerase chain reaction; reverse transcription polymerase chain reaction; trophozoite; Western blotting | - |
dc.title | DNA Topoisomerase II Is Involved in Regulation of Cyst Wall Protein Genes and Differentiation in Giardia lamblia | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1371/journal.pntd.0002218 | - |
dc.identifier.scopus | 2-s2.0-84878514953 | - |
dc.relation.journalvolume | 7 | - |
dc.relation.journalissue | 5 | - |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
crisitem.author.dept | Biochemistry and Molecular Biology | - |
crisitem.author.dept | Medical Genomics and Proteomics | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.dept | Microbiology | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.dept | Tropical Medicine and Parasitology | - |
crisitem.author.orcid | 0000-0003-0139-6513 | - |
crisitem.author.orcid | 0000-0003-0393-2340 | - |
crisitem.author.orcid | 0000-0002-8604-8085 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
crisitem.author.parentorg | College of Medicine | - |
Appears in Collections: | 生物化學暨分子生物學科研究所 |
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