https://scholars.lib.ntu.edu.tw/handle/123456789/455899
標題: | Hyaluronic acid modulates gene expression of Connective Tissue Growth Factor (CTGF), Transforming Growth Factor-β1 (TGF-β1), and Vascular Endothelial Growth Factor (VEGF) in human fibroblast-like synovial cells from advanced-stage osteoarthritis in vitro | 作者: | Lee Y.-T. Shao H.-J. JYH-HORNG WANG Liu H.-C. Hou S.-M. Tai-Horng Young |
公開日期: | 2010 | 卷: | 28 | 期: | 4 | 起(迄)頁: | 492-496 | 來源出版物: | Journal of Orthopaedic Research | 摘要: | Intraarticular injection of hyaluronan (hyaluronic acid; HA) is the common way to treat osteoarthritis (OA) of knees. This treatment cannot only maintain the viscoelastic properties of knee but also release the OA pain. However, the exact molecular mechanism is unknown. In this study, after human synovial cells were stimulated with HA and Hylan (Synvisc?) for 24 h, real-time polymerase chain reaction (real-time PCR) was used to detect the alteration of connective tissue growth factor (CTGF), transforming growth factor-β1 (TGFb1), and vascular endothelial growth factor (VEGF) gene expression, which were specific genes related to pathogenesis of OA knees. Our results illustrated that both HA and Hylan might not cause cytotoxicity or apoptosis of synovial cells in serum deprivation environment. The gene expressions of TGF-b1 and VEGF were significantly increased at the concentration of 0.1 mg/mLHA and 0.1 mg/mL Hylan, respectively (α < 0.05). The synovial cells with treatment of 0.1 mg/mL Hylan decreased the CTGF gene expression (0.66-fold) and VEGF (0.78-fold) compared to 0.1mg/mLHA(α α 0.05). We suggested that the profile of CTGF,TGF-β1, and VEGF gene expressions in our study might provide the rational mechanism for the therapeutic effect of hyaluronan on OA knees. ? 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-77949500487&doi=10.1002%2fjor.21029&partnerID=40&md5=28b6a318d058e4eb2f72bb591b14fcde https://scholars.lib.ntu.edu.tw/handle/123456789/455899 |
ISSN: | 0736-0266 | DOI: | 10.1002/jor.21029 | SDG/關鍵字: | connective tissue growth factor; hyaluronic acid; transforming growth factor beta1; vasculotropin; connective tissue growth factor; CTGF protein, human; drug derivative; hylan; immunological adjuvant; transforming growth factor beta1; vasculotropin A; VEGFA protein, human; aged; apoptosis; article; cell stimulation; cell viability; controlled study; cytotoxicity; disease severity; drug effect; drug mechanism; female; fibroblast; gene expression; human; human cell; human tissue; in vitro study; knee osteoarthritis; nucleotide sequence; pathogenesis; priority journal; real time polymerase chain reaction; serum; synoviocyte; cell culture; cell survival; fibroblast; fluorescence microscopy; gene expression; gene expression profiling; genetics; knee osteoarthritis; metabolism; pathology; radiography; synovium; Adjuvants, Immunologic; Aged; Cell Survival; Cells, Cultured; Connective Tissue Growth Factor; Female; Fibroblasts; Gene Expression; Gene Expression Profiling; Humans; Hyaluronic Acid; Microscopy, Fluorescence; Osteoarthritis, Knee; Synovial Membrane; Transforming Growth Factor beta1; Vascular Endothelial Growth Factor A |
顯示於: | 醫學系 |
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