https://scholars.lib.ntu.edu.tw/handle/123456789/456369
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | CHUN-HAN HOU | en_US |
dc.contributor.author | Chiang Y.-C. | en_US |
dc.contributor.author | Fong Y.-C. | en_US |
dc.contributor.author | Tang C.-H. | en_US |
dc.creator | Chun-Han Hou;Chiang Y.-C.;Fong Y.-C.;Tang C.-H. | - |
dc.date.accessioned | 2020-02-10T06:01:59Z | - |
dc.date.available | 2020-02-10T06:01:59Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0006-2952 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79955608916&doi=10.1016%2fj.bcp.2011.03.016&partnerID=40&md5=595d9a539748bc92b8088c6fa4539bd4 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/456369 | - |
dc.description.abstract | Chondrosarcoma is a type of highly malignant tumor with a potent capacity to invade locally and cause distant metastasis. Chondrosarcoma shows a predilection for metastasis to the lungs. WISP-1 is a cysteine-rich protein that belongs to the CCN (Cyr61, CTGF, Nov) family of matricellular proteins. However, the effect of WISP-1 on migration activity in human chondrosarcoma cells is mostly unknown. Here we found that WISP-1 increased the migration and expression of matrix metalloproteinase (MMP)-2 in human chondrosarcoma cells (JJ012 cells). We also found that human chondrosarcoma tissues had significant expression of the WISP-1 which was higher than that in normal cartilage. α5β1 monoclonal antibody and MAPK kinase (MEK) inhibitors (PD98059 and U0126) inhibited the WISP-1-induced increase of the migration and MMP-2 up-regulation of chondrosarcoma cells. WISP-1 stimulation increased the phosphorylation of focal adhesion kinase (FAK), MEK and extracellular signal-regulated kinase (ERK). In addition, NF-κB inhibitors also suppressed the cell migration and MMP-2 expression enhanced by WISP-1. Moreover, WISP-1 increased NF-κB luciferase activity and binding of p65 to the NF-κB element on the MMP-2 promoter. Taken together, our results indicated that WISP-1 enhances the migration of chondrosarcoma cells by increasing MMP-2 expression through the α5β1 integrin receptor, FAK, MEK, ERK, p65 and NF-κB signal transduction pathway. ? 2011 Elsevier Inc. All rights reserved. | - |
dc.relation.ispartof | Biochemical Pharmacology | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | CCN protein; focal adhesion kinase; gelatinase A; immunoglobulin enhancer binding protein; integrin receptor; luciferase; mitogen activated protein kinase; mitogen activated protein kinase kinase; synaptotagmin I; unclassified drug; very late activation antigen 5; WISP 1 protein; article; cancer cell culture; cell assay; cell migration; cell motility; chondrosarcoma; controlled study; enzyme activation; enzyme phosphorylation; human; human cell; metastasis potential; priority journal; promoter region; protein expression; protein function; protein protein interaction; signal transduction; upregulation; Base Sequence; Blotting, Western; Cell Line, Tumor; Cell Movement; Chondrosarcoma; Chromatin Immunoprecipitation; DNA Primers; Flow Cytometry; Humans; Integrin alpha5beta1; Intracellular Signaling Peptides and Proteins; Matrix Metalloproteinase 2; Polymerase Chain Reaction; Proto-Oncogene Proteins | - |
dc.title | WISP-1 increases MMP-2 expression and cell motility in human chondrosarcoma cells | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.bcp.2011.03.016 | - |
dc.identifier.pmid | 21453685 | - |
dc.identifier.scopus | 2-s2.0-79955608916 | - |
dc.relation.pages | 1286-1295 | - |
dc.relation.journalvolume | 81 | - |
dc.relation.journalissue | 11 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Orthopedic Surgery | - |
crisitem.author.dept | Orthopedic Surgery-NTUH | - |
crisitem.author.orcid | 0000-0002-3850-0997 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學系 |
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