https://scholars.lib.ntu.edu.tw/handle/123456789/456965
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Mo C.-F. | en_US |
dc.contributor.author | Wu F.-C. | en_US |
dc.contributor.author | Tai K.-Y. | en_US |
dc.contributor.author | Chang W.-C. | en_US |
dc.contributor.author | Chang K.-W. | en_US |
dc.contributor.author | Kuo H.-C. | en_US |
dc.contributor.author | HONG-NERNG HO | en_US |
dc.contributor.author | HSIN-FU CHEN | en_US |
dc.contributor.author | SHAU-PING LIN | en_US |
dc.creator | Mo C.-F.;Wu F.-C.;Tai K.-Y.;Chang W.-C.;Chang K.-W.;Kuo H.-C.;Ho H.-N.;Chen H.-F.;Lin S.-P. | - |
dc.date.accessioned | 2020-02-10T09:00:28Z | - |
dc.date.available | 2020-02-10T09:00:28Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 17576512 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/456965 | - |
dc.description.abstract | Introduction: Pluripotent stem cells are increasingly used to build therapeutic models, including the transplantation of neural progenitors derived from human embryonic stem cells (hESCs). Recently, long non-coding RNAs (lncRNAs), including delta-like homolog 1 gene and the type III iodothyronine deiodinase gene (DLK1-DIO3) imprinted locus-derived maternally expressed gene 3 (MEG3), were found to be expressed during neural development. The deregulation of these lncRNAs is associated with various neurological diseases. The imprinted locus DLK1-DIO3 encodes abundant non-coding RNAs (ncRNAs) that are regulated by differential methylation of the locus. We aim to study the correlation between the DLK1-DIO3-derived ncRNAs and the capacity of hESCs to differentiate into neural lineages. Methods: We classified hESC sublines into MEG3-ON and MEG3-OFF based on the expression levels of MEG3 and its downstream microRNAs as detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). A cDNA microarray was used to analyze the gene expression profiles of hESCs. To investigate the capacity of neural differentiation in MEG3-ON and MEG3-OFF hESCs, we performed neural lineage differentiation followed by neural lineage marker expression and neurite formation analyses via qRT-PCR and immunocytochemistry, respectively. MEG3-knockdown via small interfering RNA (siRNA) and small hairpin RNA (shRNA) was used to investigate the potential causative effect of MEG3 in regulating neural lineage-related gene expression. Results: DLK1-DIO3-derived ncRNAs were repressed in MEG3-OFF hESCs compared with those in the MEG3-ON hESCs. The transcriptome profile indicated that many genes related to nervous system development and neural-type tumors were differentially expressed in MEG3-OFF hESCs. Three independent MEG3-knockdown assays using different siRNA and shRNA constructs consistently resulted in downregulation of some neural lineage genes. Lower expression levels of stage-specific neural lineage markers and reduced neurite formation were observed in neural lineage-like cells derived from MEG3-OFF-associated hESCs compared with those in the MEG3-ON groups at the same time points after differentiation. Conclusions: Repression of ncRNAs derived from the DLK1-DIO3 imprinted locus is associated with reduced neural lineage differentiation potential in hESCs. ? 2015 Mo et al.; licensee BioMed Central. | - |
dc.relation.ispartof | Stem Cell Research and Therapy | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | long untranslated RNA; short hairpin RNA; small interfering RNA; transcription factor PAX6; transcriptome; eye protein; homeodomain protein; iodide peroxidase; iodothyronine deiodinase type III; long untranslated RNA; MEG3 non-coding RNA, human; microRNA; paired box transcription factor; PAX6 protein, human; repressor protein; transcription factor PAX6; transcriptome; untranslated RNA; Article; controlled study; DIO3 gene; DLK1 gene; embryo; embryonic stem cell; gene; gene control; gene expression; gene locus; genetic correlation; human; human cell; immunocytochemistry; Lentivirinae; MEG3 gene; nerve cell differentiation; nervous system development; neurite; priority journal; protein expression; reverse transcription polymerase chain reaction; RNA methylation; antagonists and inhibitors; cell differentiation; cell line; cell lineage; cytology; DNA microarray; DNA sequence; embryoid body; genetics; genome imprinting; human embryonic stem cell; immunohistochemistry; induced pluripotent stem cell; metabolism; nerve cell; real time polymerase chain reaction; RNA interference; Cell Differentiation; Cell Line; Cell Lineage; Embryoid Bodies; Eye Proteins; Genetic Loci; Genomic Imprinting; Homeodomain Proteins; Human Embryonic Stem Cells; Humans; Immunohistochemistry; Induced Pluripotent Stem Cells; Iodide Peroxidase; MicroRNAs; Neurons; Oligonucleotide Array Sequence Analysis; Paired Box Transcription Factors; PAX6 Transcription Factor; Real-Time Polymerase Chain Reaction; Repressor Proteins; RNA Interference; RNA, Long Noncoding; RNA, Small Interfering; RNA, Untranslated; Sequence Analysis, DNA; Transcriptome | - |
dc.title | Loss of non-coding RNA expression from the DLK1-DIO3 imprinted locus correlates with reduced neural differentiation potential in human embryonic stem cell lines | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1186/scrt535 | - |
dc.identifier.scopus | 2-s2.0-84928735699 | - |
dc.identifier.url | https://www2.scopus.com/inward/record.uri?eid=2-s2.0-84928735699&doi=10.1186%2fscrt535&partnerID=40&md5=37f64b14790413048adbd3e1ececc425 | - |
dc.relation.journalvolume | 6 | - |
dc.relation.journalissue | 1 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Medical Genomics and Proteomics | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Biotechnology | - |
crisitem.author.dept | Genome and Systems Biology Degree Program | - |
crisitem.author.orcid | 0000-0002-7207-0089 | - |
crisitem.author.orcid | 0000-0002-7748-4440 | - |
crisitem.author.orcid | 0000-0003-3423-991X | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Life Science | - |
顯示於: | 生物科技研究所 |
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