https://scholars.lib.ntu.edu.tw/handle/123456789/458563
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chang M.-C. | en_US |
dc.contributor.author | YU-LI CHEN | en_US |
dc.contributor.author | Lin H.-W. | en_US |
dc.contributor.author | YING-CHENG CHIANG | en_US |
dc.contributor.author | Chang C.-F. | en_US |
dc.contributor.author | Hsieh S.-F. | en_US |
dc.contributor.author | CHI-AN CHEN | en_US |
dc.contributor.author | WEI-ZEN SUN | en_US |
dc.contributor.author | WEN-FANG CHENG | en_US |
dc.creator | Chang M.-C.;Chen Y.-L.;Lin H.-W.;Chiang Y.-C.;Chang C.-F.;Hsieh S.-F.;Chen C.-A.;Sun W.-Z.;Wen-Fang Cheng | - |
dc.date.accessioned | 2020-02-14T02:49:40Z | - |
dc.date.available | 2020-02-14T02:49:40Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 1525-0016 | - |
dc.identifier.uri | 2-s2.0-85039071042 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/458563 | - |
dc.description.abstract | Ionizing radiation therapy is a well-established method of eradicating locally advanced tumors. Here, we examined whether local RT enhanced the potency of an antigen-specific DNA vaccine, and we investigated the possible underlying mechanism. Using the HPV16 E6/E7+ syngeneic TC-1 tumor, we evaluated the combination of CTGF/E7 vaccination with local irradiation with regard to synergistic antigen-specific immunity and anti-tumor effects. Tumor-bearing mice treated with local RT (6 Gy twice weekly) and CTGF/E7 DNA vaccination exhibited dramatically increased numbers of E7-specific CD8+ cytotoxic T cell precursors, higher titers of anti-E7 Abs, and significantly reduced tumor size. The combination of local RT and CTGF/E7 vaccination also elicited abscopal effects on non-irradiated local subcutaneous and distant pulmonary metastatic tumors. Local irradiation induced the expression of high-mobility group box 1 protein (HMGB-1) in apoptotic tumor cells and stimulated dendritic cell (DC) maturation, consequently inducing antigen-specific immune responses. Additionally, local irradiation eventually increased the effector-to-suppressor cell ratio in the tumor microenvironment. Overall, local irradiation enhanced the antigen-specific immunity and anti-tumor effects on local and distant metastatic tumors generated by an antigen-specific DNA vaccine. These findings suggest that the combination of irradiation with antigen-specific immunotherapy is a promising new clinical strategy for cancer therapy. Cheng and colleagues show that TC-1 tumor-bearing mice treated with local irradiation and CTGF/E7 DNA vaccination exhibited dramatically increased numbers of E7-specific CD8+ cytotoxic T cell precursors and significantly reduced tumor size. This combinational strategy also elicited abscopal effects on non-irradiated local subcutaneous and distant pulmonary metastatic tumors. ? 2017 The American Society of Gene and Cell Therapy | en_US |
dc.relation.ispartof | Molecular Therapy | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | antibody; DNA vaccine; E7 antibody; high mobility group B1 protein; unclassified drug; cancer vaccine; high mobility group B1 protein; tumor antigen; animal cell; animal experiment; animal model; animal tissue; antibody titer; antigen specificity; antineoplastic activity; apoptosis; Article; blood cell ratio; cancer immunotherapy; cancer radiotherapy; CD4+ T lymphocyte; CD8+ T lymphocyte; cell maturation; controlled study; dendritic cell; drug mechanism; drug potency; effector to supppressor cell ratio; female; immune response; lung metastasis; mouse; nonhuman; protein expression; radiation dose fractionation; regulatory mechanism; treatment duration; tumor microenvironment; tumor volume; whole body radiation; animal; cytotoxicity; disease model; drug screening; genetics; human; immunology; immunotherapy; metabolism; multimodality cancer therapy; neoplasm; pathology; procedures; regulatory T lymphocyte; tumor microenvironment; Animals; Antigens, Neoplasm; Cancer Vaccines; CD8-Positive T-Lymphocytes; Combined Modality Therapy; Cytotoxicity, Immunologic; Dendritic Cells; Disease Models, Animal; HMGB1 Protein; Humans; Immunotherapy; Mice; Neoplasms; T-Lymphocytes, Regulatory; Tumor Microenvironment; Whole-Body Irradiation; Xenograft Model Antitumor Assays | - |
dc.title | Irradiation Enhances Abscopal Anti-tumor Effects of Antigen-Specific Immunotherapy through Regulating Tumor Microenvironment | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.ymthe.2017.11.011 | - |
dc.identifier.scopus | 56601533000 | - |
dc.relation.pages | 404-419 | en_US |
dc.relation.journalvolume | 26 | en_US |
dc.relation.journalissue | 2 | en_US |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics&Gynecology-NTUHYL | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Surgery-NTUCC | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Anesthesiology | - |
crisitem.author.dept | Anesthesiology-NTUH | - |
crisitem.author.dept | Brain and Mind Sciences | - |
crisitem.author.dept | Medical Device and Imaging | - |
crisitem.author.dept | Oncology-NTUH | - |
crisitem.author.dept | Biomedical Electronics and Bioinformatics | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Oncology | - |
crisitem.author.dept | Surgery-NTUCC | - |
crisitem.author.orcid | 0000-0002-9742-4752 | - |
crisitem.author.orcid | 0000-0002-8958-5222 | - |
crisitem.author.orcid | 0000-0001-6670-7939 | - |
crisitem.author.orcid | 0000-0001-8543-2600 | - |
crisitem.author.orcid | 0000-0002-3282-6304 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital Yun-Lin Branch | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
顯示於: | 醫學系 |
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