https://scholars.lib.ntu.edu.tw/handle/123456789/458573
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chang M.-C. | en_US |
dc.contributor.author | YU-LI CHEN | en_US |
dc.contributor.author | YING-CHENG CHIANG | en_US |
dc.contributor.author | Chen T.-C. | en_US |
dc.contributor.author | Tang Y.-C. | en_US |
dc.contributor.author | CHI-AN CHEN | en_US |
dc.contributor.author | WEI-ZEN SUN | en_US |
dc.contributor.author | WEN-FANG CHENG | en_US |
dc.creator | Chang M.-C.;Chen Y.-L.;Chiang Y.-C.;Chen T.-C.;Tang Y.-C.;Chen C.-A.;Sun W.-Z.;Wen-Fang Cheng | - |
dc.date.accessioned | 2020-02-14T02:49:44Z | - |
dc.date.available | 2020-02-14T02:49:44Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0969-7128 | - |
dc.identifier.uri | 2-s2.0-84955207234 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/458573 | - |
dc.description.abstract | Ovarian cancer is a gynecologic malignancy with a high mortality rate. In the present study, we developed a novel cell-based vaccine, Meso-VAX, to generate mesothelin antigen-specific immune responses and immunotherapy against ovarian cancer. Mesothelin, a secreted protein anchored at the cell membrane, has recently been identified as a potential new tumor antigen for ovarian cancer. In this study, mice vaccinated with Meso-VAX and adeno-associated virus (AAV)-IL-12 exhibited dramatic increases in the number of mesothelin-specific CD4 + helper and CD8 + cytotoxic T-cell precursors, higher titers of anti-mesothelin Abs and in vitro tumor killing activity, and all of these mice were tumor-free after 60 days of tumor challenge. In addition, a significant reduction in peritoneal tumors and longer survival were noted in the mice vaccinated with Meso-VAX combined with AAV-IL-12. CD4 + helper and CD8 + cytotoxic T lymphocytes were essential for the antitumor effect generated by Meso-VAX combined with AAV-IL-12. The post-vaccination sera of the mice vaccinated with Meso-VAX and AAV-IL-12 also showed mesothelin-specific complement-dependent cell-mediated cytotoxicity. Our results suggest that a Meso-VAX cell-based vaccine combined with AAV-IL-12 can generate antigen-specific immunological responses and antitumor effects on ovarian cancer. ? 2016 Macmillan Publishers Limited. | en_US |
dc.relation.ispartof | Gene Therapy | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Adeno associated virus interleukin 12; cancer vaccine; meso vax; mesothelin antibody; protein antibody; tumor antigen; unclassified drug; cancer antibody; cancer vaccine; glycosylphosphatidylinositol anchored protein; interleukin 12; interleukin 2; mesothelin; tumor antigen; Adeno associated virus; animal cell; animal experiment; animal model; antibody titer; antineoplastic activity; Article; cancer immunotherapy; cancer survival; CD4 lymphocyte count; CD8+ T lymphocyte; cell membrane; drug potency; female; immune response; immunomodulation; in vitro study; mouse; nonhuman; ovary cancer; peritoneum tumor; pre T lymphocyte; priority journal; animal; blood; C57BL mouse; CD4+ T lymphocyte; cytotoxic T lymphocyte; cytotoxicity; Dependoparvovirus; genetics; human; immunology; immunotherapy; Ovarian Neoplasms; tumor cell line; vaccination; Animals; Antibodies, Neoplasm; Antigens, Neoplasm; Cancer Vaccines; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Cell Line, Tumor; Cytotoxicity, Immunologic; Dependovirus; Female; GPI-Linked Proteins; Humans; Immunotherapy; Interleukin-12; Interleukin-2; Mice; Mice, Inbred C57BL; Ovarian Neoplasms; T-Lymphocytes, Cytotoxic; Vaccination | - |
dc.title | Mesothelin-specific cell-based vaccine generates antigen-specific immunity and potent antitumor effects by combining with IL-12 immunomodulator | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1038/gt.2015.85 | - |
dc.identifier.scopus | 56601533000 | - |
dc.relation.pages | 38-49 | en_US |
dc.relation.journalvolume | 23 | en_US |
dc.relation.journalissue | 1 | en_US |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics&Gynecology-NTUHYL | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Surgery-NTUCC | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Anesthesiology | - |
crisitem.author.dept | Anesthesiology-NTUH | - |
crisitem.author.dept | Brain and Mind Sciences | - |
crisitem.author.dept | Medical Device and Imaging | - |
crisitem.author.dept | Oncology-NTUH | - |
crisitem.author.dept | Biomedical Electronics and Bioinformatics | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Oncology | - |
crisitem.author.dept | Surgery-NTUCC | - |
crisitem.author.orcid | 0000-0002-9742-4752 | - |
crisitem.author.orcid | 0000-0002-8958-5222 | - |
crisitem.author.orcid | 0000-0001-6670-7939 | - |
crisitem.author.orcid | 0000-0001-8543-2600 | - |
crisitem.author.orcid | 0000-0002-3282-6304 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital Yun-Lin Branch | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
顯示於: | 醫學系 |
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