https://scholars.lib.ntu.edu.tw/handle/123456789/458627
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chen H.-W. | en_US |
dc.contributor.author | Leng C.-H. | en_US |
dc.contributor.author | Liu H.-Y. | en_US |
dc.contributor.author | WEN-FANG CHENG | en_US |
dc.contributor.author | Chang Y.-W. | en_US |
dc.contributor.author | Wu P.-Y. | en_US |
dc.contributor.author | Lien S.-P. | en_US |
dc.contributor.author | Huang T.-Y. | en_US |
dc.contributor.author | Chiang S.-K. | en_US |
dc.contributor.author | Lin M.-H. | en_US |
dc.contributor.author | Tao M.-H. | en_US |
dc.contributor.author | Chong P. | en_US |
dc.contributor.author | Liu S.-J. | en_US |
dc.creator | Liu S.-J.;Chong P.;Tao M.-H.;Lin M.-H.;Chiang S.-K.;Huang T.-Y.;Lien S.-P.;Wu P.-Y.;Chang Y.-W.;WEN-FANG CHENG;Liu H.-Y.;Leng C.-H.;Chen H.-W. | - |
dc.date.accessioned | 2020-02-14T02:50:02Z | - |
dc.date.available | 2020-02-14T02:50:02Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1538-4047 | - |
dc.identifier.uri | 2-s2.0-73549088284 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/458627 | - |
dc.description.abstract | Identification of the cytotoxic T lymphocyte (CTL) epitopes of tumor antigens is important for effective immunotherapy. We report that a combination of epitope prediction, enzyme-linked immunosorbent assay (ELISA)-based epitope-HLA complex formation, and DNA immunization methods can improve the efficiency and accuracy of CTL epitope studies. In this study, two HLA-A11-restricted epitopes derived from human papillomavirus (HPV)18 E6 oncoprotein were identified. HLA-A11-transgenic mice immunized with these epitopes could specifically induce interferon-? (IFN?) production, cytotoxicity and peptide/HLA-A11 tetramer binding in CD8+ T-cells. To study intracellular processing of CTL epitopes, we constructed a DNA plasmid containing an endoplasmic reticulum (ER) targeting sequence as well as the HPV18 E6 and E7 genes (pEK/HPV18E6E7). CTL responses against peptide-pulsed T2/A11 cells could be detected after immunizing HLA-A11-transgenic mice with pEK/HPV18E6E7. Furthermore, the identified peptides could stimulate T-cells to secrete IFN? from HPV18-infected patients. Our results demonstrate that the antigenic E6 peptides derived from HPV18 are potential candidates for the treatment of HPV 18-associated tumors in HLA-A11+ populations. ? 2009 Landes Bioscience. | - |
dc.relation.ispartof | Cancer Biology and Therapy | - |
dc.subject | Cervical cancer; Cytotoxic T lymphocytes; HLA-A11; HPV18; Peptide-based vaccine | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | epitope; gamma interferon; HLA A11 antigen; human papillomavirus 18 e6 oncoprotein; plasmid DNA; plasmid vector; recombinant protein; tetramer; tumor antigen; unclassified drug; animal cell; animal cell culture; animal experiment; article; blood sampling; CD8+ T lymphocyte; cytokine production; cytotoxic T lymphocyte; cytotoxicity; DNA immunization; endoplasmic reticulum; enzyme linked immunosorbent assay; Human papillomavirus type 18; mouse; nonhuman; plasmid; polymerase chain reaction; protein expression; spleen cell; transgenic mouse; uterine cervix cancer | - |
dc.title | Identification of HLA-A11-restricted CTL epitopes derived from HPV type 18 using DNA immunization | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.4161/cbt.8.21.9732 | - |
dc.identifier.scopus | 56601533000 | - |
dc.relation.pages | 2025-2032 | - |
dc.relation.journalvolume | 8 | - |
dc.relation.journalissue | 21 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Oncology | - |
crisitem.author.dept | Surgery-NTUCC | - |
crisitem.author.orcid | 0000-0002-3282-6304 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
顯示於: | 醫學系 |
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