https://scholars.lib.ntu.edu.tw/handle/123456789/458631
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | CHI-AN CHEN | en_US |
dc.contributor.author | Chang M.-C. | en_US |
dc.contributor.author | WEI-ZEN SUN | en_US |
dc.contributor.author | YU-LI CHEN | en_US |
dc.contributor.author | YING-CHENG CHIANG | en_US |
dc.contributor.author | CHANG-YAO HSIEH | en_US |
dc.contributor.author | Chen S.M. | en_US |
dc.contributor.author | PO-NI HSIAO | en_US |
dc.contributor.author | WEN-FANG CHENG | en_US |
dc.creator | WEN-FANG CHENG;Hsiao P.-N.;Chen S.M.;Hsieh C.-Y.;Chiang Y.-C.;Chen Y.-L.;Sun W.-Z.;Chang M.-C.;Chen C.-A. | - |
dc.date.accessioned | 2020-02-14T02:50:03Z | - |
dc.date.available | 2020-02-14T02:50:03Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0969-7128 | - |
dc.identifier.uri | 2-s2.0-67349215725 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/458631 | - |
dc.description.abstract | Genetic immunization strategies have largely focused on the use of plasmid DNA with a gene gun. However, there remains a clear need to further improve the efficiency, safety, and cost of potential DNA vaccines. The gold particle-coated DNA format delivered through a gene gun is expensive, time and process consuming, and raises aseptic safety concerns. This study aims to determine whether a low-pressured gene gun can deliver noncarrier naked DNA vaccine without any particle coating, and generate similarly strong antigen-specific immunologic responses and potent antitumor effects compared with gold particle-coated DNA vaccine. Our results show that mice vaccinated with noncarrier naked chimeric CRT/E7 DNA lead to dramatic increases in the numbers of E7-specific CD8+ T-cell precursors and markedly raised titers of E7-specific antibodies. Furthermore, noncarrier naked CRT/E7 DNA vaccine generated potent antitumor effects against subcutaneous E7-expressing tumors and pre-established E7-expressing metastatic pulmonary tumors. In addition, mice immunized with noncarrier naked CRT/E7 DNA vaccine had significantly less burning effects on the skin compared with those vaccinated with gold particle-coated CRT/E7 DNA vaccine. We conclude that noncarrier naked CRT/E7 DNA vaccine delivered with a low-pressured gene gun can generate similarly potent immunologic responses and effective antitumor effects has fewer side effects, and is more convenient than conventional gold particle-coated DNA vaccine. | en_US |
dc.relation.ispartof | Gene Therapy | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | calreticulin; calreticulin protein E7 DNA vaccine; DNA vaccine; gold nanoparticle; naked DNA; protein E7; unclassified drug; animal cell; animal experiment; animal model; animal tissue; antigen specificity; antineoplastic activity; article; cancer gene therapy; cancer immunotherapy; cancer prevention; CD8+ T lymphocyte; chimera; controlled study; DNA immunization; drug coating; drug dosage form comparison; drug megadose; female; gene gun; immune response; injection site burning; local skin reaction; lung metastasis; mouse; neoplasm; nonhuman; priority journal; prolymphocyte; skin defect; subcutaneous tissue tumor; vaccination reaction; Animals; Antigens, CD11c; Biolistics; Buffers; Burns, Chemical; Cancer Vaccines; CD8-Positive T-Lymphocytes; Cell Line, Tumor; Cells, Cultured; Dendritic Cells; Dermis; Dose-Response Relationship, Immunologic; Drug Carriers; Enzyme-Linked Immunosorbent Assay; Epitopes, T-Lymphocyte; Female; Flow Cytometry; Gold; Immunotherapy; Injections, Intradermal; Luciferases; Mice; Mice, Inbred C57BL; Microscopy, Fluorescence; Neoplasms; Neoplasms, Experimental; Papillomavirus E7 Proteins; Pressure; Recombinant Fusion Proteins; Vaccines, DNA; Mus | - |
dc.title | Noncarrier naked antigen-specific DNA vaccine generates potent antigen-specific immunologic responses and antitumor effects | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1038/gt.2009.31 | - |
dc.identifier.scopus | 56601533000 | - |
dc.relation.pages | 776-787 | en_US |
dc.relation.journalvolume | 16 | en_US |
dc.relation.journalissue | 6 | en_US |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Anesthesiology | - |
crisitem.author.dept | Anesthesiology-NTUH | - |
crisitem.author.dept | Brain and Mind Sciences | - |
crisitem.author.dept | Medical Device and Imaging | - |
crisitem.author.dept | Oncology-NTUH | - |
crisitem.author.dept | Biomedical Electronics and Bioinformatics | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics&Gynecology-NTUHYL | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Surgery-NTUCC | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Oncology-NTUH | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Anesthesiology-NTUH | - |
crisitem.author.dept | Anesthesiology | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Oncology | - |
crisitem.author.dept | Surgery-NTUCC | - |
crisitem.author.orcid | 0000-0001-6670-7939 | - |
crisitem.author.orcid | 0000-0001-8543-2600 | - |
crisitem.author.orcid | 0000-0002-9742-4752 | - |
crisitem.author.orcid | 0000-0002-8958-5222 | - |
crisitem.author.orcid | 0000-0001-6630-0047 | - |
crisitem.author.orcid | 0000-0002-3282-6304 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital Yun-Lin Branch | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
顯示於: | 醫學系 |
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