https://scholars.lib.ntu.edu.tw/handle/123456789/458654
DC Field | Value | Language |
---|---|---|
dc.contributor.author | WEN-FANG CHENG | en_US |
dc.contributor.author | CHIEN-NAN LEE | en_US |
dc.contributor.author | Su Y.-N. | en_US |
dc.contributor.author | Chai C.-Y. | en_US |
dc.contributor.author | Chang M.-C. | en_US |
dc.contributor.author | Polo J.M. | en_US |
dc.contributor.author | Hung C.-F. | en_US |
dc.contributor.author | Wu T.-C. | en_US |
dc.contributor.author | CHANG-YAO HSIEH | en_US |
dc.contributor.author | CHI-AN CHEN | en_US |
dc.creator | Wen-Fang Cheng;Lee C.-N.;Su Y.-N.;Chai C.-Y.;Chang M.-C.;Polo J.M.;Hung C.-F.;Wu T.-C.;Hsieh C.-Y.;Chen C.-A. | - |
dc.date.accessioned | 2020-02-14T02:50:10Z | - |
dc.date.available | 2020-02-14T02:50:10Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0929-1903 | - |
dc.identifier.uri | 2-s2.0-33747280364 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/458654 | - |
dc.description.abstract | Alphavirus vectors have emerged as a promising strategy for the development of cancer vaccines and gene therapy applications. In this study, we used the replication-defective vaccine vector SIN replicon particles from a new packaging cell line (PCL) to develop SIN replicon particles encoding calreticulin (CRT) linked to a model tumor antigen, human papillomavirus type 16 (HPV16) E7 protein. The linkage of CRT to E7 in SIN replicon particles resulted in a significant increase in E7-specific CD8+ T-cell precursors and a strong antitumor effect against E7-expressing tumors in vaccinated mice. SINrep5-CRT/E7 replicon particles enhanced presentation of E7 through the major histocompatibility complex (MHC) class I pathway by infecting dendritic cells (DCs) directly and pulsing DCs with lysates of cells infected by SINrep5-CRT/E7 replicons. Vaccination of immunocompromised (BALB/c nu/nu) mice with SINrep5-CRT/E7 replicon particles also generated significant reduction of lung tumor nodules, suggesting that antiangiogenesis may contribute to the antitumor effect of SINrep5-CRT/E7 replicon particles. Furthermore, SINrep5-CRT/E7 replicon particles generated long-term in vivo tumor protection effects and antigen-specific memory immunities. We concluded that the CRT strategy used in the context of SIN replicon particles facilitated the generation of a highly effective vaccine for cancer prophylaxis and immunotherapy. ? 2006 Nature Publishing Group All rights reserved. | en_US |
dc.relation.ispartof | Cancer Gene Therapy | en_US |
dc.subject.other | calreticulin; cancer vaccine; major histocompatibility antigen class 1; protein E7; tumor antigen; virus vector; Alpha virus; angiogenesis; animal cell; animal experiment; antigen specificity; antineoplastic activity; article; cancer immunotherapy; cell lysate; cellular immunity; controlled study; dendritic cell; female; Human papillomavirus type 16; immunological memory; memory cell; nonhuman; priority journal; replicon; Sindbis virus; T lymphocyte activation; tumor immunity; virus particle; Analysis of Variance; Animals; Calreticulin; Cancer Vaccines; CD8-Positive T-Lymphocytes; Cell Line, Tumor; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; Gene Therapy; Human papillomavirus 16; Humans; Immunologic Memory; Immunotherapy; Lung Neoplasms; Mice; Mice, Inbred C57BL; Papillomavirus E7 Proteins; Replicon; Sindbis Virus; Alphavirus; Human papillomavirus type 16; Sindbis virus | - |
dc.subject.other | [SDGs]SDG3 | - |
dc.title | Sindbis virus replicon particles encoding calreticulin linked to a tumor antigen generate long-term tumor-specific immunity | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1038/sj.cgt.7700956 | - |
dc.identifier.scopus | 56601533000 | - |
dc.relation.pages | 873-885 | en_US |
dc.relation.journalvolume | 13 | en_US |
dc.relation.journalissue | 9 | en_US |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Oncology | - |
crisitem.author.dept | Surgery-NTUCC | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics&Gynecology-NTUHHC | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Oncology-NTUH | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.orcid | 0000-0002-3282-6304 | - |
crisitem.author.orcid | 0000-0002-1725-0407 | - |
crisitem.author.orcid | 0000-0001-6670-7939 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | NTU Hsin-Chu Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
Appears in Collections: | 醫學系 |
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