https://scholars.lib.ntu.edu.tw/handle/123456789/458666
Title: | Induction of human papillomavirus type 16-specific immunologic responses in a normal and an human papillomavirus-infected populations | Authors: | WEN-FANG CHENG CHIEN-NAN LEE Su Y.-N. Chang M.-C. Hsiao W.-C. CHI-AN CHEN CHANG-YAO HSIEH |
Issue Date: | 2005 | Journal Volume: | 115 | Journal Issue: | 1 | Start page/Pages: | 136-149 | Source: | Immunology | Abstract: | Human papillomavirus (HPV) infection, especially with the oncogenic genotypes, is the most important risk factor for developing cervical cancer. We focused on generating HPV16 E7-specific cytotoxic CD8+ T lymphocytes and evaluating HPV16 E7-specific immune responses in HPV16-infected and uninfected populations. Peripheral blood mononuclear cells (PBMCs) were first collected from an uninfected group with an human lymphocyte antigen (HLA) A2 haplotype (four volunteers). Mature monocyte-derived dendritic cells (DCs) were generated from the PBMCs and pulsed with one of two HLA-A2-restricted E7 peptides, aa 11-20 [YMLDLQPETT] and aa 86-93 [TLGIVCPI], as antigen presenting cells. The autologous na?ve or cultured PBMCs were then cultured with peptide-pulsed DCs to detect the HPV16 E7-specific immune responses by a variety of techniques such as enzyme-linked immunosorbent assay (ELISA), enzyme-linked immunospot (ELISPOT) assay and cytotoxic T lymphocyte assay. Interferon-γ (IFN-γ) from E7-specific cytotoxic CD8+ T lymphocytes stimulated with the respective peptide was detected by ELISA. Using ELISPOT analysis, a marked increase in the number of IFN-γ-secreting CD8 + E7-specific lymphocytes was observed following peptide stimulation. Cultured CD8+ T lymphocytes were highly cytotoxic against the CaSki cells. PBMCs were then colleted from an HPV16-infected population of the HLA-A2 haplotype, including four persons of HPV16 infection only, four with cervical intraepithelial neoplasia (CIN) lesions, and four cervical cancer patients. We then compared the immunologie responses to E7 between HPV16-infected and uninfected populations by ELISA and ELISPOT assay. The E7-specific immunologic responses of the HPV16-infected populations were significantly higher than those of the uninfected population. In addition, persons with an HPV16 infection only or those with CIN lesions generated higher E7-specific immunologic responses than cervical cancer patients. Our results demonstrate methods for evaluating E7-sepcific immunologic responses and reflect the biological responses of HPV16-infected people during different periods of cervical disease. ? 2005 Blackwell Publishing Ltd. |
URI: | 2-s2.0-17844404289 https://scholars.lib.ntu.edu.tw/handle/123456789/458666 |
ISSN: | 0019-2805 | DOI: | 10.1111/j.1365-2567.2005.02126.x | SDG/Keyword: | gamma interferon; HLA A2 antigen; amino acid sequence; article; cellular immunity; controlled study; cytotoxic T lymphocyte; cytotoxicity; dendritic cell; enzyme linked immunosorbent assay; enzyme linked immunospot assay; female; human; human cell; Human papillomavirus type 16; in vitro study; peripheral blood mononuclear cell; priority journal; protein secretion; uterine cervix cancer; uterine cervix carcinoma in situ; virus infection; CD8-Positive T-Lymphocytes; Cells, Cultured; Cervical Intraepithelial Neoplasia; Cytotoxicity, Immunologic; Dendritic Cells; Enzyme-Linked Immunosorbent Assay; Epitopes, T-Lymphocyte; Female; Haplotypes; HLA-A2 Antigen; Humans; Interferon Type II; Papillomaviridae; Papillomavirus Infections; Peptide Fragments; Uterine Cervical Neoplasms |
Appears in Collections: | 醫學系 |
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