https://scholars.lib.ntu.edu.tw/handle/123456789/458666
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | WEN-FANG CHENG | en_US |
dc.contributor.author | CHIEN-NAN LEE | en_US |
dc.contributor.author | Su Y.-N. | en_US |
dc.contributor.author | Chang M.-C. | en_US |
dc.contributor.author | Hsiao W.-C. | en_US |
dc.contributor.author | CHI-AN CHEN | en_US |
dc.contributor.author | CHANG-YAO HSIEH | en_US |
dc.creator | Wen-Fang Cheng;Lee C.-N.;Su Y.-N.;Chang M.-C.;Hsiao W.-C.;Chen C.-A.;Hsieh C.-Y. | - |
dc.date.accessioned | 2020-02-14T02:50:15Z | - |
dc.date.available | 2020-02-14T02:50:15Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0019-2805 | - |
dc.identifier.uri | 2-s2.0-17844404289 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/458666 | - |
dc.description.abstract | Human papillomavirus (HPV) infection, especially with the oncogenic genotypes, is the most important risk factor for developing cervical cancer. We focused on generating HPV16 E7-specific cytotoxic CD8+ T lymphocytes and evaluating HPV16 E7-specific immune responses in HPV16-infected and uninfected populations. Peripheral blood mononuclear cells (PBMCs) were first collected from an uninfected group with an human lymphocyte antigen (HLA) A2 haplotype (four volunteers). Mature monocyte-derived dendritic cells (DCs) were generated from the PBMCs and pulsed with one of two HLA-A2-restricted E7 peptides, aa 11-20 [YMLDLQPETT] and aa 86-93 [TLGIVCPI], as antigen presenting cells. The autologous na?ve or cultured PBMCs were then cultured with peptide-pulsed DCs to detect the HPV16 E7-specific immune responses by a variety of techniques such as enzyme-linked immunosorbent assay (ELISA), enzyme-linked immunospot (ELISPOT) assay and cytotoxic T lymphocyte assay. Interferon-γ (IFN-γ) from E7-specific cytotoxic CD8+ T lymphocytes stimulated with the respective peptide was detected by ELISA. Using ELISPOT analysis, a marked increase in the number of IFN-γ-secreting CD8 + E7-specific lymphocytes was observed following peptide stimulation. Cultured CD8+ T lymphocytes were highly cytotoxic against the CaSki cells. PBMCs were then colleted from an HPV16-infected population of the HLA-A2 haplotype, including four persons of HPV16 infection only, four with cervical intraepithelial neoplasia (CIN) lesions, and four cervical cancer patients. We then compared the immunologie responses to E7 between HPV16-infected and uninfected populations by ELISA and ELISPOT assay. The E7-specific immunologic responses of the HPV16-infected populations were significantly higher than those of the uninfected population. In addition, persons with an HPV16 infection only or those with CIN lesions generated higher E7-specific immunologic responses than cervical cancer patients. Our results demonstrate methods for evaluating E7-sepcific immunologic responses and reflect the biological responses of HPV16-infected people during different periods of cervical disease. ? 2005 Blackwell Publishing Ltd. | - |
dc.relation.ispartof | Immunology | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | gamma interferon; HLA A2 antigen; amino acid sequence; article; cellular immunity; controlled study; cytotoxic T lymphocyte; cytotoxicity; dendritic cell; enzyme linked immunosorbent assay; enzyme linked immunospot assay; female; human; human cell; Human papillomavirus type 16; in vitro study; peripheral blood mononuclear cell; priority journal; protein secretion; uterine cervix cancer; uterine cervix carcinoma in situ; virus infection; CD8-Positive T-Lymphocytes; Cells, Cultured; Cervical Intraepithelial Neoplasia; Cytotoxicity, Immunologic; Dendritic Cells; Enzyme-Linked Immunosorbent Assay; Epitopes, T-Lymphocyte; Female; Haplotypes; HLA-A2 Antigen; Humans; Interferon Type II; Papillomaviridae; Papillomavirus Infections; Peptide Fragments; Uterine Cervical Neoplasms | - |
dc.title | Induction of human papillomavirus type 16-specific immunologic responses in a normal and an human papillomavirus-infected populations | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1111/j.1365-2567.2005.02126.x | - |
dc.identifier.scopus | 56601533000 | - |
dc.relation.pages | 136-149 | - |
dc.relation.journalvolume | 115 | - |
dc.relation.journalissue | 1 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Oncology | - |
crisitem.author.dept | Surgery-NTUCC | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics&Gynecology-NTUHHC | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Oncology-NTUH | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.orcid | 0000-0002-3282-6304 | - |
crisitem.author.orcid | 0000-0002-1725-0407 | - |
crisitem.author.orcid | 0000-0001-6670-7939 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | NTU Hsin-Chu Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學系 |
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