https://scholars.lib.ntu.edu.tw/handle/123456789/458690
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Hsu K.-F. | en_US |
dc.contributor.author | Hung C.-F. | en_US |
dc.contributor.author | WEN-FANG CHENG | en_US |
dc.contributor.author | He L. | en_US |
dc.contributor.author | Slater L.A. | en_US |
dc.contributor.author | Ling M. | en_US |
dc.contributor.author | Wu T.-C. | en_US |
dc.creator | Hsu K.-F.;Hung C.-F.;Wen-Fang Cheng;He L.;Slater L.A.;Ling M.;Wu T.-C. | - |
dc.date.accessioned | 2020-02-14T02:50:25Z | - |
dc.date.available | 2020-02-14T02:50:25Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0969-7128 | - |
dc.identifier.uri | 2-s2.0-0035077790 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/458690 | - |
dc.description.abstract | Naked DNA vaccines represent an attractive approach for generating antigen-specific immunity because of their stability and simplicity of delivery. There are particular concerns with DNA vaccines however, such as potential integration into the host genome, cell transformation, and limited potency. The usage of DNA-based alphaviral RNA replicons (suicidal DNA vectors) may alleviate the concerns of integration or transformation since suicidal DNA vectors eventually cause lysis of transfected cells. To improve further the potency of suicidal DNA vaccines, we evaluated the effect of linking Mycobacterium tuberculosis heat shock protein 70 (Hsp70) to human papillomavirus type 16 (HPV-16) E7 as a model antigen on antigen-specific immunity generated by a DNA-based Semliki Forest virus (SFV) RNA vector, pSCA1. Our results indicated that this suicidal DNA vaccine containing E7/Hsp70 fusion genes generated significantly higher E7-specific T cell-mediated immune responses than vaccines containing the wild-type E7 gene in vaccinated mice. More importantly, this fusion converted a less effective vaccine into one with significant potency against established E7-expressing metastatic tumors. The antitumor effect was predominantly CD8-dependent. These results indicate that linkage of Hsp70 to the antigen may greatly enhance the potency of suicidal DNA vaccines. | - |
dc.relation.ispartof | Gene Therapy | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | antibiotic agent; CD8 antigen; DNA vaccine; heat shock protein 70; virus protein; virus RNA; Alpha virus; animal experiment; antigen specificity; antineoplastic activity; article; cell transformation; cellular immunity; controlled study; cytolysis; DNA vector; drug delivery system; drug potency; drug stability; female; fusion gene; genetic transfection; host; immunity; metastasis; mouse; Mycobacterium tuberculosis; nonhuman; priority journal; Semliki Forest alphavirus; suicide gene; vaccination; Wart virus; Animals; Antibodies, Viral; Antigens, Viral; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Cell Line; Female; HSP70 Heat-Shock Proteins; Humans; Immunity, Cellular; Lung Neoplasms; Mice; Mice, Inbred C57BL; Mycobacterium tuberculosis; Oncogene Proteins, Viral; Papillomaviridae; Semliki forest virus; Vaccination; Vaccines, DNA; Actinobacteria (class); Alphavirus; Animalia; DNA viruses; Human papillomavirus; Human papillomavirus type 16; Human papillomavirus types; Mycobacterium; Mycobacterium tuberculosis; Semliki Forest virus; uncultured actinomycete | - |
dc.title | Enhancement of suicidal DNA vaccine potency by linking Mycobacterium tuberculosis heat shock protein 70 to an antigen | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1038/sj.gt.3301408 | - |
dc.identifier.scopus | 56601533000 | - |
dc.relation.pages | 376-383 | - |
dc.relation.journalvolume | 8 | - |
dc.relation.journalissue | 5 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Oncology | - |
crisitem.author.dept | Surgery-NTUCC | - |
crisitem.author.orcid | 0000-0002-3282-6304 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
顯示於: | 醫學系 |
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