https://scholars.lib.ntu.edu.tw/handle/123456789/458816
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | MING-KWANG SHYU | en_US |
dc.contributor.author | RAY-HWANG YUAN | en_US |
dc.contributor.author | JIN-CHUNG SHIH | en_US |
dc.contributor.author | MU-ZON WU | en_US |
dc.contributor.author | HUEY-LING CHEN | en_US |
dc.contributor.author | Kuo Y.-C | en_US |
dc.contributor.author | CHUNG-LIANG CHIEN | en_US |
dc.contributor.author | LU-PING CHOW | en_US |
dc.contributor.author | Chen H.-L | en_US |
dc.contributor.author | FON-JOU HSIEH | en_US |
dc.creator | Ming-Kwang Shyu;Yuan R.-H;Shih J.-C;Wu M.-Z;Chen H.-L;Kuo Y.-C;Chien C.-L;Chow L.-P;Chen H.-L;Hsieh F.-J. | - |
dc.date.accessioned | 2020-02-14T05:49:42Z | - |
dc.date.available | 2020-02-14T05:49:42Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1590-8658 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/458816 | - |
dc.description.abstract | Background and aims: To evaluate donor cell engraftment and the kinetics of cell repopulation in the injured mouse liver following human umbilical cord blood cell transplantation. Methods: Nonobese diabetic/severe immunodeficient mice were treated with allyl alcohol to induce liver injury. Twenty-four hours later, umbilical cord blood derived mononuclear cells were transplanted by intra-splenic injection. Mice were sacrificed from 1 to 180 days after transplantation. Temporal changes in the ratio of human cells and fluorescence counts of human sex-determining region Y alleles in mouse liver were determined to evaluate the kinetics of cell repopulation. Mouse liver and sera were examined for the presence of human albumin. Results: Human cell repopulation was extremely rapid in the first week following transplantation, with a doubling time of 1.16-1.39 days apparent. Thereafter cell doubling rate slowed significantly. Cells displaying characteristics of human hepatocytes were still evident at 180 days. Human albumin was detected in mouse liver and sera. Conclusion: These findings confirm those from previous studies demonstrating that cells derived from human umbilical cord blood have the capacity to differentiate into cells with human hepatocyte characteristics in mouse liver following injury. Moreover, the detailed information collected regarding the kinetics of human cell repopulation in mouse liver will be of relevance to future studies examining the use of umbilical cord blood cells in liver transplantation therapy. ? 2007 Editrice Gastroenterologica Italiana S.r.l. | en_US |
dc.relation.ispartof | Digestive and Liver Disease | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | albumin; allyl alcohol; DNA; testis determining factor; albumin blood level; allele; animal cell; animal tissue; article; cell count; cell differentiation; controlled study; cord blood stem cell transplantation; DNA sequence; female; fluorescence analysis; histopathology; human; human cell; liver cell; liver injury; mononuclear cell; mouse; nonhuman; nonobese diabetic mouse; population dynamics; SCID mouse; sequence analysis; sex determination; tissue distribution; umbilical cord blood; Animals; Cell Differentiation; Female; Fetal Blood; Genes, sry; Humans; Liver; Liver Transplantation; Mice; Mice, Inbred NOD; Polymerase Chain Reaction; Serum Albumin; Stem Cells; Time Factors | - |
dc.title | Kinetics and functional assay of liver repopulation after human cord blood transplantation | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.dld.2007.01.007 | - |
dc.identifier.pmid | 17379589 | - |
dc.identifier.scopus | 2-s2.0-33947727478 | - |
dc.relation.pages | 455-465 | en_US |
dc.relation.journalvolume | 39 | en_US |
dc.relation.journalissue | 5 | en_US |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | National Taiwan University Hospital Yun-Lin Branch | - |
crisitem.author.dept | Obstetrics&Gynecology-NTUHYL | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | Surgery | - |
crisitem.author.dept | NTU BioMedical Park Hospital | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics&Gynecology-NTUHHC | - |
crisitem.author.dept | Forensic Medicine | - |
crisitem.author.dept | Pathology-NTUH | - |
crisitem.author.dept | Anatomy and Cell Biology | - |
crisitem.author.dept | Medical Genomics and Proteomics | - |
crisitem.author.dept | Biochemistry and Molecular Biology | - |
crisitem.author.orcid | 0000-0001-9057-4101 | - |
crisitem.author.orcid | 0000-0002-2767-633X | - |
crisitem.author.orcid | 0000-0002-0296-4327 | - |
crisitem.author.orcid | 0000-0002-7509-1643 | - |
crisitem.author.orcid | 0000-0002-2572-0595 | - |
crisitem.author.orcid | 0000-0001-9806-486X | - |
crisitem.author.orcid | 0000-0002-4420-211X | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital Yun-Lin Branch | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital Hsin-Chu Branch | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | NTU Hsin-Chu Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學系 |
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